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Ume6D_BY4741 on expression/splicing microarrays

ABSTRACT: ume6D_BY4741 vegetative RNA compared to WT_BY4741 vegetative RNA. ume6D haploid was generated in the BY4741 strain background (ATCC: 201388) by PCR-mediated cassette-based gene replacement where the UME6 ORF was replaced with KANMX6, the deletion was verified by PCR. Vegetatively grown; Dye swap; Reference vegetative WT_BY4741

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Lily Shiue

PROVIDER: E-GEOD-24680 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Integration of a splicing regulatory network within the meiotic gene expression program of Saccharomyces cerevisiae.

Munding Elizabeth M EM Igel A Haller AH Shiue Lily L Dorighi Kristel M KM Treviño Lisa R LR Ares Manuel M

Genes & development 20101201 23

Splicing regulatory networks are essential components of eukaryotic gene expression programs, yet little is known about how they are integrated with transcriptional regulatory networks into coherent gene expression programs. Here we define the MER1 splicing regulatory network and examine its role in the gene expression program during meiosis in budding yeast. Mer1p splicing factor promotes splicing of just four pre-mRNAs. All four Mer1p-responsive genes also require Nam8p for splicing activation ...[more]

PMID: 21123654

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mer1D_SK1, mer2D_SK1, mer3D_SK1, spo22D_SK1, and spo70D_SK1 on expression/splicing microarrays

Project description:mer1D_SK1, mer2D_SK1, mer3D_SK1, spo22D_SK1, spo70D_SK1 late 9h meiotic time point. mer1D, mer2D, mer3D, spo22D diploid, and spo70D diploid was generated in the SK1 strain background (ATCC: MYA-2089) by PCR-mediated cassette-based gene replacement where the MER1, MER2, MER3, SPO22, or SPO70 ORF respectively was replaced with HIS3 from S. kluyveri then the transformants were sporulated and the homozygous deletion was verified by PCR. Meiotic time point =9h; Dye swap; Reference WT_SK1 9h

2011-04-05 | E-GEOD-24678 | biostudies-arrayexpress

ume6D_BY4741 on expression/splicing microarrays

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Saccharomyces cerevisiae

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| PRJNA85249 | ENA

Transcription profiling of S. cerevisiae (budding yeast) ume6 deletion strains vs wild-type in different media to determine the complement of Ume6 dependent genes during mitosis and meiosis

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