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Sequence-Targeted Nucleosome Sliding in vivo - Nucleosome Mapping

ABSTRACT: Nucleosome positions were determined in wild type cells, cells lacking Isw2 or Ume6, and cells containing a hybrid Chd1-Ume6 chimeric remodeler

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE72570 | GEO | 2016/03/12

SECONDARY ACCESSION(S): PRJNA294376

REPOSITORIES: GEO

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Sequence-Targeted Nucleosome Sliding in vivo - Nucleosome Mapping

Project description:Nucleosome positions were determined in wild type cells, cells lacking Isw2 or Ume6, and cells containing a hybrid Chd1-Ume6 chimeric remodeler Matched MNase digests from W303 strain variants during log growth (OD600=0.4-0.6) were subject to paired-end sequencing for nucleosome mapping. For effects of the engineered fusion remodeling protein, a catalytically inactive (ATPase dead D513N) variant was included as a control.

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Sequence-Targeted Nucleosome Sliding in vivo

Project description:This SuperSeries is composed of the SubSeries listed below.

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Sequence-Targeted Nucleosome Sliding in vivo

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-03-12 | E-GEOD-72572 | biostudies-arrayexpress

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Project description:RNA sequencing was performed on various W303 variants to determine effects of nucleosome repositioning on transcript abundance RNA sequencing was carried out in multiple backgrounds to determine effects of nucleosome repositioning in various contexts. For engineered chromatin remodeling factors, a catalytically inactive control was included.

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Isw2 and Ume6 ChIP-chip in transcription factor deletion (M-NM-^Tume6, M-NM-^Tnrg1, M-NM-^Tcin5, and M-NM-^Tsok2) and DNA-looping deficient (sua7-1) cells

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