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Transcription profiling of untreated, HIV-1 vector-infected and TNFalpha-treated human Jurkat T cells

ABSTRACT: Jurkat T cells (in triplicate per treatment group) were left untreated in culture, infected with VSV-G-pseudotyped HIV-based vector (which transduces Tat and eGFP) or treated with TNFalpha.

ORGANISM(S): Homo sapiens

SUBMITTER: Mary Lewinski

PROVIDER: E-GEOD-2504 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide analysis of chromosomal features repressing human immunodeficiency virus transcription.

Lewinski M K MK Bisgrove D D Shinn P P Chen H H Hoffmann C C Hannenhalli S S Verdin E E Berry C C CC Ecker J R JR Bushman F D FD

Journal of virology 20050601 11

We have investigated regulatory sequences in noncoding human DNA that are associated with repression of an integrated human immunodeficiency virus type 1 (HIV-1) promoter. HIV-1 integration results in the formation of precise and homogeneous junctions between viral and host DNA, but integration takes place at many locations. Thus, the variation in HIV-1 gene expression at different integration sites reports the activity of regulatory sequences at nearby chromosomal positions. Negative regulation ...[more]

PMID: 15890899

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