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Transcriptional Dominance of Pax7 in Adult Myogenesis Is Due to High-Affinity Recognition of Homeodomain Motifs

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE25064: ChIP-Seq of Pax7 and Pax3 in myoblasts GSE32266: Mouse Myoblast Pax3, Pax7 overexpression and control Refer to individual Series

ORGANISM(S): Mus musculus

SUBMITTER: OGIC Ontario Genomics Innovation Centre (OGIC)

PROVIDER: E-GEOD-25092 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional dominance of Pax7 in adult myogenesis is due to high-affinity recognition of homeodomain motifs.

Soleimani Vahab D VD Punch Vincent G VG Kawabe Yoh-ichi Y Jones Andrew E AE Palidwor Gareth A GA Porter Christopher J CJ Cross Joe W JW Carvajal Jaime J JJ Kockx Christel E M CE van IJcken Wilfred F J WF Perkins Theodore J TJ Rigby Peter W J PW Grosveld Frank F Rudnicki Michael A MA

Developmental cell 20120517 6

Pax3 and Pax7 regulate stem cell function in skeletal myogenesis. However, molecular insight into their distinct roles has remained elusive. Using gene expression data combined with genome-wide binding-site analysis, we show that both Pax3 and Pax7 bind identical DNA motifs and jointly activate a large panel of genes involved in muscle stem cell function. Surprisingly, in adult myoblasts Pax3 binds a subset (6.4%) of Pax7 targets. Despite a significant overlap in their transcriptional network, P ...[more]

PMID: 22609161

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