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ChIP-Seq of Pax7 and Pax3 in myoblasts

ABSTRACT: Examination of binding locations of Pax3 and Pax7 in primary myoblasts UCSC track hub available at: http://www.ogic.ca/projects/Soleimani_2012_Pax7_hub/hub.txt For details on viewing the track hub in the UCSC Genome Browser: http://altair.dartmouth.edu/ucsc/goldenPath/help/hgTrackHubHelp.html#View 3 Samples (Control, Pax7 ChIP, Pax3 ChIP)

ORGANISM(S): Mus musculus

SUBMITTER: OGIC Ontario Genomics Innovation Centre (OGIC)

PROVIDER: E-GEOD-25064 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional dominance of Pax7 in adult myogenesis is due to high-affinity recognition of homeodomain motifs.

Soleimani Vahab D VD Punch Vincent G VG Kawabe Yoh-ichi Y Jones Andrew E AE Palidwor Gareth A GA Porter Christopher J CJ Cross Joe W JW Carvajal Jaime J JJ Kockx Christel E M CE van IJcken Wilfred F J WF Perkins Theodore J TJ Rigby Peter W J PW Grosveld Frank F Rudnicki Michael A MA

Developmental cell 20120517 6

Pax3 and Pax7 regulate stem cell function in skeletal myogenesis. However, molecular insight into their distinct roles has remained elusive. Using gene expression data combined with genome-wide binding-site analysis, we show that both Pax3 and Pax7 bind identical DNA motifs and jointly activate a large panel of genes involved in muscle stem cell function. Surprisingly, in adult myoblasts Pax3 binds a subset (6.4%) of Pax7 targets. Despite a significant overlap in their transcriptional network, P ...[more]

PMID: 22609161

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