Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: Keith Szulwach
PROVIDER: E-GEOD-25398 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Song Chun-Xiao CX Szulwach Keith E KE Fu Ye Y Dai Qing Q Yi Chengqi C Li Xuekun X Li Yujing Y Chen Chih-Hsin CH Zhang Wen W Jian Xing X Wang Jing J Zhang Li L Looney Timothy J TJ Zhang Baichen B Godley Lucy A LA Hicks Leslie M LM Lahn Bruce T BT Jin Peng P He Chuan C
Nature biotechnology 20101212 1
In contrast to 5-methylcytosine (5-mC), which has been studied extensively, little is known about 5-hydroxymethylcytosine (5-hmC), a recently identified epigenetic modification present in substantial amounts in certain mammalian cell types. Here we present a method for determining the genome-wide distribution of 5-hmC. We use the T4 bacteriophage β-glucosyltransferase to transfer an engineered glucose moiety containing an azide group onto the hydroxyl group of 5-hmC. The azide group can be chemi ...[more]