Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from WT or p65-/- mouse embryonic fibroblasts (MEFs) infected with Toxoplasma gondii.

ABSTRACT: Toxoplasma strains have been shown to modulate host cell transcription. We have found a type II Toxoplasma gene, GRA15, which activates the nuclear translocation of the NF-kappaB p65 transcription factor. We used microarrays to determine how GRA15II modulates host cell transcription, and whether this transcription is dependent on the p65 transcription factor. WT or p65-/- MEFs were infected with type I (RH), type I GRA15II (RH GRA15II), type II (Pru), or type II GRA15KO (Pru GRA15KO) parasites for 18-24 hours. Total RNA was isolated and hybridized to Affymetrix GeneChip Human Genome U133A 2.0 arrays.

ORGANISM(S): Mus musculus

SUBMITTER: Emily Rosowski 

PROVIDER: E-GEOD-25469 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Strain-specific activation of the NF-kappaB pathway by GRA15, a novel Toxoplasma gondii dense granule protein.

Rosowski Emily E EE   Lu Diana D   Julien Lindsay L   Rodda Lauren L   Gaiser Rogier A RA   Jensen Kirk D C KD   Saeij Jeroen P J JP  

The Journal of experimental medicine 20110103 1

NF-κB is an integral component of the immune response to Toxoplasma gondii. Although evidence exists that T. gondii can directly modulate the NF-κB pathway, the parasite-derived effectors involved are unknown. We determined that type II strains of T. gondii activate more NF-κB than type I or type III strains, and using forward genetics we found that this difference is a result of the polymorphic protein GRA15, a novel dense granule protein which T. gondii secretes into the host cell upon invasio  ...[more]

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