Dataset Information

Human WIPI interactome (LC-MSMS)

ABSTRACT: Analysis of the WIPI protein interactome using nanoLC-MS/MS analysis upon the immunopurification of GFP-tagged WIPI1, WIPI2B, WIPI2D, WIPI3, WIPI4 and GFP alone from U2OS cells. Prior to immunopurifications, cells were either kept under fed (DMEM/FCS) or starved conditions (EBSS), or were treated with wortmannin (200 nM) for 3 h.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Bone

DISEASE(S): Osteosarcoma

SUBMITTER: Nicolas Nalpas

LAB HEAD: Tassula Proikas-Cezanne

PROVIDER: PXD006119 | Pride | 2017-06-01

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	0287_0316_txt.zip	Other
	0539_txt.zip	Other
	20111116_CO_0287TaPC_R07_TiO2.RAW	Raw
	20111116_CO_0287TaPC_R08_TiO2.RAW	Raw
	20111116_CO_0287TaPC_R09_TiO2.RAW	Raw

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Publications

WIPI3 and WIPI4 β-propellers are scaffolds for LKB1-AMPK-TSC signalling circuits in the control of autophagy.

Bakula Daniela D Müller Amelie J AJ Zuleger Theresia T Takacs Zsuzsanna Z Franz-Wachtel Mirita M Thost Ann-Katrin AK Brigger Daniel D Tschan Mario P MP Frickey Tancred T Robenek Horst H Macek Boris B Proikas-Cezanne Tassula T

Nature communications 20170531

Autophagy is controlled by AMPK and mTOR, both of which associate with ULK1 and control the production of phosphatidylinositol 3-phosphate (PtdIns3P), a prerequisite for autophagosome formation. Here we report that WIPI3 and WIPI4 scaffold the signal control of autophagy upstream of PtdIns3P production and have a role in the PtdIns3P effector function of WIPI1-WIPI2 at nascent autophagosomes. In response to LKB1-mediated AMPK stimulation, WIPI4-ATG2 is released from a WIPI4-ATG2/AMPK-ULK1 comple ...[more]

PMID: 28561066

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