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Unknown,Transcriptomics,Genomics,Proteomics

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Genomic Profiling of HMGN1 Reveals an Association with Chromatin at Regulatory Regions

ABSTRACT: High mobility group (HMG) proteins interact with other architectural proteins to regulate chromatin structure. We performed genome-wide mapping experiments to examine the distribution of HMGN1 across the human genome. We discovered that HMGN1 proteins are enriched at regulatory regions such as DNase I HS sites, distal transcription factor binding sites and promoters of actively transcribed genes. YY1, the Yin Yang 1 transcription factor, is a ubiquitous transcription factor that functions either as a transcriptional activator or a repressor, depending on its interacting partner. To test the preferential localization of HMGN1 with active regulatory regions, we examined the association of transcription factor YY1 and HMGN1 in CD4+ T cells. Examination of the genomic profile of HMGN1 and the relationship with regulatory elements in CD4+ T cells.

ORGANISM(S): Homo sapiens

SUBMITTER: Dustin Schones

PROVIDER: E-GEOD-25674 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genomic profiling of HMGN1 reveals an association with chromatin at regulatory regions.

Cuddapah Suresh S Schones Dustin E DE Cui Kairong K Roh Tae-Young TY Barski Artem A Wei Gang G Rochman Mark M Bustin Michael M Zhao Keji K

Molecular and cellular biology 20101220 4

The interaction of architectural proteins such as the linker histone H1 and high-mobility-group (HMG) proteins with nucleosomes leads to changes in chromatin structure and histone modifications and alters the cellular transcription profile. The interaction of HMG proteins with chromatin is dynamic. However, it is not clear whether the proteins are constantly and randomly redistributed among all the nucleosomes or whether they preferentially associate with, and turn over at, specific regions in c ...[more]

PMID: 21173166

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Genomic Profiling of HMGN1 Reveals an Association with Chromatin at Regulatory Regions

Project description:High mobility group (HMG) proteins interact with other architectural proteins to regulate chromatin structure. We performed genome-wide mapping experiments to examine the distribution of HMGN1 across the human genome. We discovered that HMGN1 proteins are enriched at regulatory regions such as DNase I HS sites, distal transcription factor binding sites and promoters of actively transcribed genes. YY1, the Yin Yang 1 transcription factor, is a ubiquitous transcription factor that functions either as a transcriptional activator or a repressor, depending on its interacting partner. To test the preferential localization of HMGN1 with active regulatory regions, we examined the association of transcription factor YY1 and HMGN1 in CD4+ T cells.

2010-11-30 | GSE25674 | GEO

YY1 is a structural regulator of enhancer-promoter loops [HiChIP]

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