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Effect of downregulation of the high grain protein content (GPC) gene on the wheat transcriptome during monocarpic senescence

ABSTRACT: When GPC transcription factor in hexaploid wheat is down-regulated by stable RNA interference (GPC RNAi), senescence is significantly delayed and grain protein content together with the overall nutrient partitioning to the grain is greatly reduced. mRNA-seq was used to catalogue the genes that are regulated by the GPC transcription factor during monocarpic senescence. cDNA was prepared from wild type bread wheat plants and GPC RNAi plants 12 days after anthesis and sequenced by Illumina. Four biological replications per genotypes were sequenced. To determine gene expression levels, reads were aligned to a reference transcriptome generated by assemblying 454-reads obtained from the same biological material (454 assembled sequences: TSA project 59945 - accession numbers: HP608076-HP639668).

ORGANISM(S): Triticum aestivum

SUBMITTER: Dario Cantu

PROVIDER: E-GEOD-25759 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Effect of the down-regulation of the high Grain Protein Content (GPC) genes on the wheat transcriptome during monocarpic senescence.

Cantu Dario D Pearce Stephen P SP Distelfeld Assaf A Christiansen Michael W MW Uauy Cristobal C Akhunov Eduard E Fahima Tzion T Dubcovsky Jorge J

BMC genomics 20111007

<h4>Background</h4>Increasing the nutrient concentration of wheat grains is important to ameliorate nutritional deficiencies in many parts of the world. Proteins and nutrients in the wheat grain are largely derived from the remobilization of degraded leaf molecules during monocarpic senescence. The down-regulation of the NAC transcription factor Grain Protein Content (GPC) in transgenic wheat plants delays senescence (>3 weeks) and reduces the concentration of protein, Zn and Fe in the grain (>3 ...[more]

PMID: 21981858

Similar Datasets

Effect of downregulation of the high grain protein content (GPC) gene on the wheat transcriptome during monocarpic senescence

Project description:When GPC transcription factor in hexaploid wheat is down-regulated by stable RNA interference (GPC RNAi), senescence is significantly delayed and grain protein content together with the overall nutrient partitioning to the grain is greatly reduced. mRNA-seq was used to catalogue the genes that are regulated by the GPC transcription factor during monocarpic senescence.

2011-09-23 | GSE25759 | GEO

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2010-05-29 | GSE22048 | GEO

Small RNAs in transgenic TaNAM-RNAi wheat plants

2010-05-28 | E-GEOD-22048 | biostudies-arrayexpress

Transcription profiling of wheat GF-transgenic-B102,1-1 vs control-L88-31 lines endosperm tissue (14 and 28 days post anthesis-dpa) and in leaf tissue (8 days post germination ?dpg)

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2005-10-05 | E-MEXP-306 | biostudies-arrayexpress

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2014-07-18 | E-MTAB-2137 | biostudies-arrayexpress

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Regulation of Zn and Fe transporters by the GPC1 gene during wheat monocarpic senescence

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2014-12-23 | E-GEOD-60635 | biostudies-arrayexpress

Tissue layers from developing wheat grain at 12 days post-anthesis

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2011-11-08 | E-MTAB-861 | biostudies-arrayexpress

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