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Effect of downregulation of the high grain protein content (GPC) gene on the wheat transcriptome during monocarpic senescence

ABSTRACT: When GPC transcription factor in hexaploid wheat is down-regulated by stable RNA interference (GPC RNAi), senescence is significantly delayed and grain protein content together with the overall nutrient partitioning to the grain is greatly reduced. mRNA-seq was used to catalogue the genes that are regulated by the GPC transcription factor during monocarpic senescence.

ORGANISM(S): Triticum aestivum

PROVIDER: GSE25759 | GEO | 2011/09/23

SECONDARY ACCESSION(S): PRJNA134053

REPOSITORIES: GEO

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Effect of downregulation of the high grain protein content (GPC) gene on the wheat transcriptome during monocarpic senescence

Project description:When GPC transcription factor in hexaploid wheat is down-regulated by stable RNA interference (GPC RNAi), senescence is significantly delayed and grain protein content together with the overall nutrient partitioning to the grain is greatly reduced. mRNA-seq was used to catalogue the genes that are regulated by the GPC transcription factor during monocarpic senescence. cDNA was prepared from wild type bread wheat plants and GPC RNAi plants 12 days after anthesis and sequenced by Illumina. Four biological replications per genotypes were sequenced. To determine gene expression levels, reads were aligned to a reference transcriptome generated by assemblying 454-reads obtained from the same biological material (454 assembled sequences: TSA project 59945 - accession numbers: HP608076-HP639668).

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