Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression data from mouse Nuocytes

ABSTRACT: Nuocytes are a recently described cell that responds to both IL-25 and IL-33 and produce high levels of IL-13 and IL-5 Mice were administered with 3 daily doses of IL-25 (400 ng/dose). Nuocytes were purified by flow cytometry. Spleen cells prepared from IL-25-treated mice were depleted of lineage+ cells before cell sorting by incubation with biotin-conjugated anti-CD3, anti-CD19, anti-CD11b and anti-FcεRI antibodies before removal of antibody-bound cells by magnetic separation using Dynabeads (Invitrogen). Lineage-depleted cells were stained with PE-conjugated antibodies against CD4, CD8, B220, TER-119 and CD11b, a FITC-conjugated antibody against CD45, and an APC-conjugated antibody against ICOS. PE−, FITC+, APC+ cells were collected using a Mo-flo cell sorter, and purity was checked by staining with lineage antibodies and antibodies against IL-17BR and T1/ST2. Following purification nuocytes were prepared directly for microarray gene expression analysis or cultured with IL-33 and IL-7 (both at 10 nanograms/ml) for 9 days before being prepared for microarray gene expression analysis.

ORGANISM(S): Mus musculus

SUBMITTER: Andrew MCKENZIE

PROVIDER: E-GEOD-25890 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Nuocytes represent a new innate effector leukocyte that mediates type-2 immunity.

Neill Daniel R DR Wong See Heng SH Bellosi Agustin A Flynn Robin J RJ Daly Maria M Langford Theresa K A TK Bucks Christine C Kane Colleen M CM Fallon Padraic G PG Pannell Richard R Jolin Helen E HE McKenzie Andrew N J AN

Nature 20100303 7293

Innate immunity provides the first line of defence against invading pathogens and provides important cues for the development of adaptive immunity. Type-2 immunity-responsible for protective immune responses to helminth parasites and the underlying cause of the pathogenesis of allergic asthma-consists of responses dominated by the cardinal type-2 cytokines interleukin (IL)4, IL5 and IL13 (ref. 5). T cells are an important source of these cytokines in adaptive immune responses, but the innate cel ...[more]

PMID: 20200518

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Dataset Information

Expression data from mouse Nuocytes

Publications

Nuocytes represent a new innate effector leukocyte that mediates type-2 immunity.

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