Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouseWT vs CD137L-/- GC B cells

ABSTRACT: WT and CD137L-/- mice of 8-12 weeks old were immunized intra-peritoneally with 50 µg chicken gamma- globulin conjugated to 4-hydroxy-3-nitrophenylacetyl (NP-CG) in alum or infected intranasally with 25 hemagglutinin units of influenza virus strain A/NT/60/68 in 50 µl HBSS. Nine days after immunization or infection, B cells were enriched from pooled spleens and lymph nodes of 4 mice per test group by means of magnetic labelled bead cell separation (MACS) using anti-mouse BD ImagTM CD45R/B220-particles DM. The B220+ MACS-sorted populations were stained with anti-GL7-FITC and anti-CD19-PE to sort CD19+GL7+ GC and CD19+GL7- non-GC B lymphocytes by flow cytometry on a FACSAria (BD). Around 1 million cells of each sample were obtained. These cells were spun down and the pellet was frozen in liquid nitrogen and kept at -80 ºC before RNA extraction.

ORGANISM(S): Mus musculus

SUBMITTER: Victor Peperzak 

PROVIDER: E-NCMF-29 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mice deficient for CD137 ligand are predisposed to develop germinal center-derived B-cell lymphoma.

Middendorp Sabine S   Xiao Yanling Y   Song Ji-Ying JY   Peperzak Victor V   Krijger Peter H L PH   Jacobs Heinz H   Borst Jannie J  

Blood 20090716 11

In the germinal center (GC), B cells proliferate dramatically and diversify their immunoglobulin genes, which increases the risk of malignant transformation. The GC B-cell reaction relies on crosstalk with follicular dendritic cells (FDCs), to which the costimulatory receptor CD137 on FDCs and its ligand on GC B cells potentially contribute. We report that mice deficient for CD137 ligand (CD137L) are predisposed to develop B-cell lymphoma, with an incidence of approximately 60% at 12 months of a  ...[more]

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