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Gene expression-based analysis of neural tube closure for the posterior neuropore of Fkbp8 embryos at E9.5

ABSTRACT: Three wildtype and three nullizygous embryonic posterior neural tube tissues were collected at embryonic day 9.5 and the gene expression patterns were profiled on CodeLink Mouse Whole Genome Bioarrays. Wild type versus nullizygous Fkbp8 embryonic tissues were compared individually and as separate groups to determine classifiers and differences in expression due to the nullizygous mutation.

ORGANISM(S): Mus musculus

SUBMITTER: Robert Cabrera

PROVIDER: E-GEOD-25974 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression-based analysis of neural tube closure for the posterior neuropore of Splotch embryos at E9.5

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Gene expression-based analysis of neural tube closure for the posterior neuropore

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2010-12-10 | E-GEOD-25983 | biostudies-arrayexpress

Gene expression-based analysis of neural tube closure for the posterior neuropore of Splotch embryos at E9.5

2010-12-10 | GSE25975 | GEO

Gene expression-based analysis of neural tube closure for the posterior neuropore of Fkbp8 embryos at E9.5

2010-12-10 | GSE25974 | GEO

RNA-seq of mouse individual epithelial somites through development

Project description:Somites are transient embryonic structures consisting of hundreds of cells that bud off from the anterior tip of the presomitic mesoderm on each side of the neural tube. They give rise to the vertebrae and associated skeletal muscles, nerves, and blood vessels. To characterise the transcriptional changes that orchestrate mouse somitogenesis, we have generated coupled RNA-seq and ATAC-seq profiles of individual somites, across embryonic development. We collected the three most posterior pairs of somites, which correspond to those most recently segmented, from embryos containing 8, 18, 21, 25, 27 and 35 pairs of somites. The three somites are labelled I, II and III from the most posterior to the most anterior. From each pair, one somite was used for RNA-seq and the other one for ATAC-seq.

2023-01-24 | E-MTAB-12511 | biostudies-arrayexpress

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2023-01-24 | E-MTAB-12539 | biostudies-arrayexpress

Transcription profiling of reduced folate carrier (RFC1-/-) mouse knockouts at E9.5 reveals altered gene expression of the cubulin-megalin complex

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2008-04-05 | E-GEOD-10659 | biostudies-arrayexpress

GLI3 Controls the Proliferative Expansion of Digit Progenitors and Exit toward Chondrogenesis to Constrain the Autopod to Pentadactyly

Project description:The transcriptome of the anterior handplates of mouse limb buds of Gli3 constitutive and Hoxa13-Cre Gli3 conditional mutant embryos was compared to the transcriptome of limb buds of wild type and Gli3 heterozygote embryos at embryonic day E11.75. All samples carried one copy of the Hoxa13-Cre allele.

2012-03-01 | E-MEXP-3495 | biostudies-arrayexpress

MicroRNA profiling of mouse embryo gonad during the critical period of sex determination

Project description:Expression profiling of micro RNAs in mouse embryonic gonads before (11.5 days post coitum) and after (13.5 days post coitum) the critical period of sex determination

2012-05-11 | E-MEXP-2252 | biostudies-arrayexpress

Transcriptome profiling at neural tube closure site

Project description:Maternal diabetes is a teratogen that can lead to neural tube closure defects in the offspring. We therefore sought to compare gene expression profiles at the site of neural tube closure between stage-matched embryos from normal dams, and embryos from diabetic dams. Neurulation-stage mouse embryos at 8.5 days of gestation were used to prepare neural tissue at the anterior aspect of neural tube closure site 1. Tissue was procured from the open neural tube immediately anterior of the closure site, and from the closed neural tube immediately posterior to the closure site by laser microdissection. For each sample, 10 sections were pooled, total RNA was extracted, and 7 ng of total RNA were used for expression profiling by Tag sequencing using an Applied Biosystems SolidSAGE kit for library construction, and an AB SOLiD 5500 XL instrument for sequencing. Sequence reads were mapped to RefSeq RNA, and count data per gene were obtained using a modified version of the Applied Biosystems SOLiDâ?¢ SAGEâ?¢ Analysis Software. diabetic dam - closed neural tube // diabetic dam - open neural tube // normal dam - closed neural tube // normal dam - open neural tube

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