Unknown,Transcriptomics,Genomics,Proteomics

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SAGE analysis of A. thaliana leaf tissue

ABSTRACT: Arabidopsis thaliana (Col-4) aerial tissue collected from 14-day old plants. Grown under sterile conditions at 22 degrees Celsius and 125 uE light on 0.5xMS media. This GEO Series was created by the GEO staff as part of a cleanup effort to ensure that all GEO Samples are included within a Series entry.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Dustin Cram 

PROVIDER: E-GEOD-25993 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Maximizing the efficacy of SAGE analysis identifies novel transcripts in Arabidopsis.

Robinson Stephen J SJ   Cram Dustin J DJ   Lewis Christopher T CT   Parkin Isobel A P IA  

Plant physiology 20041001 2

The efficacy of using Serial Analysis of Gene Expression (SAGE) to analyze the transcriptome of the model dicotyledonous plant Arabidopsis was assessed. We describe an iterative tag-to-gene matching process that exploits the availability of the whole genome sequence of Arabidopsis. The expression patterns of 98% of the annotated Arabidopsis genes could theoretically be evaluated through SAGE and using an iterative matching process 79% could be identified by a tag found at a unique site in the ge  ...[more]

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