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SAGE analysis of cardiac fibroblasts isolated from adult mouse heart

ABSTRACT: Cardiac fibroblasts were isolated from 3-month old C57BL/6 mice. RNA was prepared from quiescent, serum-starved cardiac fibroblasts in passage 15. A total number of 118654 tags was sequenced. After removal of duplicate ditags and linker-derived sequences 110169 tags remained. This GEO Series was created by the GEO staff as part of a cleanup effort to ensure that all GEO Samples are included within a Series entry.

ORGANISM(S): Mus musculus

SUBMITTER: Albert Smolenski

PROVIDER: E-GEOD-25757 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Quantitative analysis of the cardiac fibroblast transcriptome-implications for NO/cGMP signaling.

Smolenski Albert A Schultess Jan J Danielewski Oliver O Garcia Arguinzonis Maisa I MI Thalheimer Petra P Kneitz Susanne S Walter Ulrich U Lohmann Suzanne M SM

Genomics 20040401 4

Cardiac fibroblasts regulate tissue repair and remodeling in the heart. To quantify transcript levels in these cells we performed a comprehensive gene expression study using serial analysis of gene expression (SAGE). Among 110,169 sequenced tags we could identify 30,507 unique transcripts. A comparison of SAGE data from cardiac fibroblasts with data derived from total mouse heart revealed a number of fibroblast-specific genes. Cardiac fibroblasts expressed a specific collection of collagens, mat ...[more]

PMID: 15028281

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