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Transcriptome analysis of Bacillus cereus B3711 during silver ion stress condition

ABSTRACT: We have employed a whole genome microarray for gene expression profilling to understand the global view of the cellular mechanism and metabolic response of B. cereus B3711 exposed to 1 mM silver nitrate. RNA was extracted from B. cereus grown under silver treated and untreated set of conditions in duplicates at two different time points (30 & 60 min). Comparing the transcript profile of the control and treated cells, several differentially expressed genes were identified. These includes genes involved in various metabolic pathways, such as arginine and alanine metabolism, membrane transport system and alternate respiratory proteins such as arsenate reductase, stress related proteins, proteins of transcriptional regulators, hypothetical proteins and protein of two-component systems. Agilent one-color experiment,Organism: Bacillus cereus ,Agilent-023971 Genotypic designed Custom Bacillus cereus 8x15k , Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)

ORGANISM(S): Bacillus cereus

SUBMITTER: Genotypic technology

PROVIDER: E-GEOD-26043 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome analysis of Bacillus cereus B3711 during silver ion stress condition

Project description:We have employed a whole genome microarray for gene expression profilling to understand the global view of the cellular mechanism and metabolic response of B. cereus B3711 exposed to 1 mM silver nitrate. RNA was extracted from B. cereus grown under silver treated and untreated set of conditions in duplicates at two different time points (30 & 60 min). Comparing the transcript profile of the control and treated cells, several differentially expressed genes were identified. These includes genes involved in various metabolic pathways, such as arginine and alanine metabolism, membrane transport system and alternate respiratory proteins such as arsenate reductase, stress related proteins, proteins of transcriptional regulators, hypothetical proteins and protein of two-component systems.

2010-12-14 | GSE26043 | GEO

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Project description:RNA-seq experiment comparing the transcriptomes of Bacillus cereus G9241 WT to B. cereus G9241 ∆pBCXO1 when cultured both 37 and 25 degree celsius. B. cereus G9241 is a B. cereus sensu stricto strain that was isolated from a welder with and anthrax-like illness. B. cereus G9241 carries the plasmids pBCXO1 and pBC210. pBCX01 has 99.6% sequence identity to pXO1 carried by Bacillus anthracis and encodes the tripartite anthrax toxin genes and atxA, a mammalian virulence transcriptional regulator. B. cereus G9241 WT and B. cereus G9241 ∆pBCXO1 were cultured to exponential phase at either 37 or 25 degree celsius before samples were taken for RNA extraction, library prep and sequencing.

2023-05-13 | E-MTAB-12883 | biostudies-arrayexpress

Distinct roles of ComK1 and ComK2 in gene regulation in Bacillus cereus (overexpressed ComK)

Project description:Comparison of the Bacillus cereus with overexpressed Bacillus subtilis ComK (Bacillus cereus pNWcomKBsu) vs Bacillus cereus carrying empty plasmid (Bacillus cereus pNW33N) One condition design comparision of (IPTG induced overexpression construct vs IPTG induced empty plasmid) including a dye swap, 3 biological replicate

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postnatal fluoxetine evoked gene expression changes in the rat hippocampus

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2013-12-01 | E-GEOD-42940 | biostudies-arrayexpress

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