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Distinct roles of ComK1 and ComK2 in gene regulation in Bacillus cereus (overexpressed ComK)

ABSTRACT: Comparison of the Bacillus cereus with overexpressed Bacillus subtilis ComK (Bacillus cereus pNWcomKBsu) vs Bacillus cereus carrying empty plasmid (Bacillus cereus pNW33N) One condition design comparision of (IPTG induced overexpression construct vs IPTG induced empty plasmid) including a dye swap, 3 biological replicate

ORGANISM(S): Bacillus cereus

SUBMITTER: Akos Kovacs

PROVIDER: E-GEOD-27265 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Distinct roles of ComK1 and ComK2 in gene regulation in Bacillus cereus.

Mirończuk Aleksandra M AM Maňu Amagoia A Kuipers Oscar P OP Kovács Akos T AT

PloS one 20110701 7

The B. subtilis transcriptional factor ComK regulates a set of genes coding for DNA uptake from the environment and for its integration into the genome. In previous work we showed that Bacillus cereus expressing the B. subtilis ComK protein is able to take up DNA and integrate it into its own genome. To extend our knowledge on the effect of B. subtilis ComK overexpression in B. cereus we first determined which genes are significantly altered. Transcriptome analysis showed that only part of the c ...[more]

PMID: 21747963

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