Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene Expression Analysis of Fezf1 Mutant Main Olfactory Epithelium

ABSTRACT: Mice lacking the transcription factor Fezf1 exhibit defects in the structural and molecular organiztion of their olfactory system. To invetigate this at the level of gene expression, we isolated Fezf1 expressing cells by FACS from the MOE of Fezf1+/- or Fezf1-/- animals and compared their gene expression profiles. The targeted Fezf1 allele contains an EGFP coding sequence under control of the endogenous Fezf1 promoter. We utilized this to isolate Fezf1+/- or Fezf1 -/- cells from the microdissected MOE tissue of E18.5 mouse embryos. Total RNA was isolated from these cells and gene expression examined by Affymetrix 1.0 ST array.

ORGANISM(S): Mus musculus

SUBMITTER: Matthew Eckler

PROVIDER: E-GEOD-26064 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Fezf1 and Fezf2 are required for olfactory development and sensory neuron identity.

Eckler Matthew J MJ McKenna William L WL Taghvaei Sahar S McConnell Susan K SK Chen Bin B

The Journal of comparative neurology 20110701 10

The murine olfactory system consists of main and accessory systems that perform distinct and overlapping functions. The main olfactory epithelium (MOE) is primarily involved in the detection of volatile odorants, while neurons in the vomeronasal organ (VNO), part of the accessory olfactory system, are important for pheromone detection. During development, the MOE and VNO both originate from the olfactory pit; however, the mechanisms regulating development of these anatomically distinct organs fr ...[more]

PMID: 21452247

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Dataset Information

Gene Expression Analysis of Fezf1 Mutant Main Olfactory Epithelium

Publications

Fezf1 and Fezf2 are required for olfactory development and sensory neuron identity.

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