Whole Transcriptome Amplification for Gene Expression Profiling and Development of Molecular Archives
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Arul Chinnaiyan
PROVIDER: E-GEOD-2618 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Whole transcriptome amplification for gene expression profiling and development of molecular archives.
Tomlins Scott A SA Mehra Rohit R Rhodes Daniel R DR Shah Rajal B RB Rubin Mark A MA Bruening Eric E Makarov Vladimir V Chinnaiyan Arul M AM
Neoplasia (New York, N.Y.) 20060201 2
Expression profiling of clinically obtainable tumor specimens has been hindered by the need for microgram quantities of RNA. In vitro transcription (IVT)-based amplifications are most commonly used to amplify small quantities of RNA for microarray analysis. However, significant drawbacks exist with IVT-based amplification, and the need for alternative amplification methods remains. Herein, we validate whole transcriptome amplification (WTA), an exponential amplification technique that produces c ...[more]
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