Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Intestine mRNA tagging

ABSTRACT: mRNA tagging in the intestine of C. elegans. FLAG::PAB-1 was expressed from the intestine-specific promoter for ges-1 and used to immunoprecipitate FLAG::PAB-1/mRNA complexes from the intestine with anti-FLAG antibody on agarose beads. This mRNA was amplified, cDNA was synthesized and labeled with Cy5. Total RNA from the same strain, but without the IP was amplified and cDNA was synthesized and labeled with Cy3. These two samples were compared on C. elegans DNA printed arrays from the Stuart Kim lab. 1938 intestine-expressed genes were identified (p<0.001) after eight repeats of the experiment A replicate experimental design type is where a series of replicates are performed to evaluate reproducibility or as a pilot study to determine the appropriate number of replicates for a subsequent experiments. Type = replicate_design Series_overall_design: Computed

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Min Jiang

PROVIDER: E-GEOD-2626 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Chromosomal clustering and GATA transcriptional regulation of intestine-expressed genes in C. elegans.

Pauli Florencia F Liu Yueyi Y Kim Yoona A YA Chen Pei-Jiun PJ Kim Stuart K SK

Development (Cambridge, England) 20051214 2

We used mRNA tagging to identify genes expressed in the intestine of C. elegans. Animals expressing an epitope-tagged protein that binds the poly-A tail of mRNAs (FLAG::PAB-1) from an intestine-specific promoter (ges-1) were used to immunoprecipitate FLAG::PAB-1/mRNA complexes from the intestine. A total of 1938 intestine-expressed genes (P<0.001) were identified using DNA microarrays. First, we compared the intestine-expressed genes with those expressed in the muscle and germline, and identifie ...[more]

PMID: 16354718

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Dataset Information

Intestine mRNA tagging

Publications

Chromosomal clustering and GATA transcriptional regulation of intestine-expressed genes in C. elegans.

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