Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Two tobacco transgenic lines over-expressing grapevine polygalacturonase-inhibiting protein (Vvpgip1) vs. WT tobacco under normal growth conditions

ABSTRACT: Polygalacturonase-inhibiting proteins (PGIPs) in plant cell walls inhibit fungal endopolygalacturonases (ePGs). Their action directly limits the effective ingress of the pathogen and is thought to lead to an up-regulated defense response. We previously showed that, PGIP activity, ePG inhibition and decreased Botrytis cinerea susceptibility were correlated in tobacco plants over-expressing grapevine (Vitis vinifera) Vvpgip1. In order to evaluate the base-line impact of PGIP over-expression, the transcriptome and hormone profiles of two transgenic lines with characterized resistance phenotypes were investigated in the absence of pathogen infection. Three microarray slides in total were hybridized: two slides using Vvpgip1 line 37 and WT, with a dye swap included to account for dye bias; and one slide using Vvpgip1 line 45 as test and WT as reference.

ORGANISM(S): Nicotiana tabacum

SUBMITTER: Erik Alexandersson

PROVIDER: E-GEOD-26324 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco.

Alexandersson Erik E Becker John Vw JV Jacobson Dan D Nguema-Ona Eric E Steyn Cobus C Denby Katherine J KJ Vivier Melané A MA

BMC research notes 20111113

<h4>Background</h4>Polygalacturonase-inhibiting proteins (PGIPs) directly limit the effective ingress of fungal pathogens by inhibiting cell wall-degrading endopolygalacturonases (ePGs). Transgenic tobacco plants over-expressing grapevine (Vitis vinifera) Vvpgip1 have previously been shown to be resistant to Botrytis infection. In this study we characterized two of these PGIP over-expressing lines with known resistance phenotypes by gene expression and hormone profiling in the absence of pathoge ...[more]

PMID: 22078230

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Dataset Information

Two tobacco transgenic lines over-expressing grapevine polygalacturonase-inhibiting protein (Vvpgip1) vs. WT tobacco under normal growth conditions

Publications

Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco.

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