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Analysis of the early transcriptome signature of Nipah virus infection in primary endothelial cells

ABSTRACT: Nipah virus (NiV) is a recently emerged zoonotic Paramyxovirus that causes regular outbreaks in East Asia with mortality rate exceeding 75%. Major cellular targets of NiV infection are endothelial cells and neurons. To better understand virus-host interaction, we analysed the transcriptome profile of NiV infection in primary human umbilical vein endothelial cells. We found that NiV infection strongly induces genes involved in interferon response in endothelial cells. Among the top ten upregulated genes, we identified the chemokine CXCL10 (interferon-induced protein 10, IP-10), an important chemoattractant involved in the generation of inflammatory immune response and neurotoxicity. We performed microarray gene expression profiling of NiV infected HUVEC cell (2 replicates) and of uninfected HUVEC cell (2 replicates).

ORGANISM(S): Homo sapiens

SUBMITTER: HORVAT Branka

PROVIDER: E-GEOD-32902 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Lethal Nipah virus infection induces rapid overexpression of CXCL10.

Mathieu Cyrille C Guillaume Vanessa V Sabine Amélie A Ong Kien Chai KC Wong Kum Thong KT Legras-Lachuer Catherine C Horvat Branka B

PloS one 20120229 2

Nipah virus (NiV) is a recently emerged zoonotic Paramyxovirus that causes regular outbreaks in East Asia with mortality rate exceeding 75%. Major cellular targets of NiV infection are endothelial cells and neurons. To better understand virus-host interaction, we analyzed the transcriptome profile of NiV infection in primary human umbilical vein endothelial cells. We further assessed some of the obtained results by in vitro and in vivo methods in a hamster model and in brain samples from NiV-inf ...[more]

PMID: 22393386

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Analysis of the early transcriptome signature of infection of primary endothelial cells by Nipah virus vs. Nipah virus deleted for the expression of non-structural C protein

Project description:Nipah virus (NiV) is a highly pathogenic, negative strand RNA paramyxovirus that has recently emerged from flying foxes to cause serious human disease. To study the poorly-understood role of nonstructural NiV proteins in NiV pathogenesis, we generated recombinant viruse lacking the expression of accesory NiV C protein (NiVM-bM-^HM-^FC). To analyse the molecular basis of NiVM-bM-^HM-^FC attenuation we have used the gene microarray approach to study early changes of gene expression in infected primary human endothelial cells, which is a major cellular target of human NiV infection. We performed microarray gene expression profiling of NiV infected HUVEC cell (2 replicates), of uninfected HUVEC cell (2 replicates) and of NiVM-bM-^HM-^FC infected HUVEC cell (2 replicates).

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2011-03-16 | E-GEOD-22812 | biostudies-arrayexpress

Analysis of the early transcriptome signature of Nipah virus infection in primary endothelial cells

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2012-08-24 | GSE32902 | GEO

CCN5, a novel transcriptional repressor

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2011-11-30 | E-GEOD-25431 | biostudies-arrayexpress

gene expression profiling in CD69 positive blood cells selected from Healthy blood Donors and HTLV-1-infected patients

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