Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mouse ventilator-induced lung injury

ABSTRACT: Isolated perfused and ventilated BALB/c mouse lung model 3 conditions c-10cmH2O pressure ventilation 3h, LPS-10cmH2O pressure ventilation 3h in the presence of E. coli LPS in the perfusion buffer (3mg/kg), ov-overventilation with 25cmH2O pressure 3h

ORGANISM(S): Mus musculus

SUBMITTER: Tamas Dolinay

PROVIDER: E-GEOD-2635 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

mouse ventilator-induced lung injury

Project description:Isolated perfused and ventilated BALB/c mouse lung model 3 conditions c-10cmH2O pressure ventilation 3h, LPS-10cmH2O pressure ventilation 3h in the presence of E. coli LPS in the perfusion buffer (3mg/kg), ov-overventilation with 25cmH2O pressure 3h Keywords: parallel sample

2005-05-10 | GSE2635 | GEO

Mouse ventilator-induced lung injury experiment

Project description:Gene expression analysis of BALB/c mouse lung tissue following LPS, high pressure ventilation and LPS+high pressure ventilation treatment Keywords: VILI, gene expression profiling, ARDS

2006-02-12 | GSE4215 | GEO

Transcription profiling of mouse Ventilator-induced lung injury in C57BL\6 strain

Project description:This study was undertaken to examine differential gene expression across the whole genome during short-term ventilator-induced lung injury in mice, a commonly used model of acute lung injury, as compared with spontaneous ventilation. Experiment Overall Design: Mice were anesthetized with isoflurane followed by ketamine/xylaxine. Saline (0.25 ml) was given every hour ip. A tracheotomy tube was placed and the mice were ventilated with an initial peak airway pressure of 20 cmH2O approximating a tidal volume of 20 ml/kg and without end-expiratory pressure. Ventilation was continued for 3h. Tidal volume was not adjusted. Body temperature was monitored with a digital rectal thermometer and maintained at 37C with a heating table and external heating lamp. Control mice were treated identically, but were not mechanically ventilated (i.e. breathed spontaneously). There were 5 biological relicates in each group.

2008-06-20 | E-GEOD-11434 | biostudies-arrayexpress

Age Dependent Gene Expression Responses to Inhaled Lipopolysaccharide Combined with Mechanical Ventilation

Project description:Children have a lower incidence and mortality from acute lung injury than adults, and infections are the most common event associated with acute lung injury (ALI). To study the effects of age on susceptibility to ALI, we investigated the responses to microbial products combined with mechanical ventilation in juvenile (21 day) and adult (16 week-old) mice. We hypothesized that the increased incidence and severity of lung injury associated with increasing age is due in large part to acquired changes in the way in which inflammatory responses are activated in the lungs in response to microbial products and mechanical ventilation. Juvenile (21 day) and adult (16 week) C57BL/6 mice were treated with an aerosol of E. coli 0111:B4 lipopolysaccharide (LPS) (20 mL of 0.1 mg/mL) for 30 minutes in a sealed aerosol chamber, immediately followed by mechanical ventilation (LPS+MV) using tidal volume = 15 mL/kg, rate = 80 breaths/min, FiO2 = 30% and positive end expiratory pressure = 2 cm H2O for the duration of the study period time = 2 hours. Comparison groups included mice treated with LPS or mechanical ventilation (MV) alone, and untreated age-matched controls. There were N = 4 animals per group except the juvenile mice treated with MV alone and LPS+MV where there were N = 3. Each sample was an individual animal, therefore there were 30 samples. Mice treated with LPS alone were placed into a sealed aerosol chamber as stated above, and then allowed to breath spontaneously with free access to food and water for the duration of the study period time = 2 hours. Mice treated with MV alone were treated with the mechanical ventilation protocol stated above for the duration of the study period time = 2 hours. At the end of the study period, the mice were euthanized, and the lungs were immediately removed and placed into RNAlater (Ambion, Austin, TX) for at least 24 hr prior to isolation of total lung mRNA.

2009-11-23 | E-GEOD-18341 | biostudies-arrayexpress

The effect of goal-directed fluid therapy combined with lung protective ventilation strategy on the cardiopulmonary function of patients undergoing laparoscopic radical resection of colorectal cancer

Project description:Interventions: TG group:GDFT combined with traditional ventilation;PG group:GDFT and LPS Primary outcome(s): Respiratory dynamics index Study Design: Parallel

| 2716233 | ecrin-mdr-crc

IRG1 Inflammasome Proteomics Experiments

Project description:BMDMs of indicated phenotypes were stimulated with LPS for 24h and analyzed or restimulated with LPS for additional 3h and analyzed. In some experiments 1mM itaconate was added at 4h of LPS first stimulation.

2021-09-09 | PXD020089 | Pride

Transcriptome analysis of in vitro endotoxin tolerant human monocytes

Project description:Monocyte exposure to lipopolysaccharide (LPS) induces a transient state in which these cells are refractory to further endotoxin stimulation. Here we demonstrate the transcriptome analysis of in vitro generated LPS refractory monocytes upon subsequent re-exposure to LPS for different time points. Total RNA obtained from human monocyte exposure treated with LPS for 3h, 6h, or 24h, subsequent to endotoxin tolerization or not.

2009-03-13 | E-GEOD-15219 | biostudies-arrayexpress

GR and BRG1 cistromes of murine primary bone marrow derived macrophages treated with LPS and Dex+LPS [ChIP-seq]

Project description:We mapped the genome wide binding profile of the chromatin remodeller BRG1 in wild type bone marrow derived macrophages (BMDMs) after 3h LPS and LPS plus Dex treatment by ChIPseq. Additionally, we profiled the GR cistrome in Dex + LPS-treated BMDMs after 3h treatment by ChIPseq.

2023-06-23 | GSE186512 | GEO

Expression data from intact and ventilation-preconditioned murine lungs

Project description:To study the effects of previous exposure to mechanical ventilation may modify the development of Ventilator-induced lung injury, preconditioning was induced by low-pressure ventilation for 90 minutes. After 1 week, intact (sham) and preconditioned mice were sacrificed, the lungs extracted and gene expression measured in order to identify differences responsible for the observed tolerance to ventilator-induced lung injury observed in preconditioned animals. 6 samples were analyzed, from 3 intact (sham) and 3 preconditioned CD1 mice.

2015-08-21 | E-GEOD-65783 | biostudies-arrayexpress

Mus musculus

Project description:Mechanical Ventilation Augments LPS-induced Lung Injury

| PRJNA91623 | ENA