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Microarray profiling of monocytic differentiaion reveals miRNA-mRNA intrinsic correlation (miRNA)

ABSTRACT: Since the expression profile of miRNAs is specific in different tissues or under different physiological conditions, the correlations between miRNAs and mRNAs could vary under different biological circumstances. This is a study also used expression profiles of miRNA and mRNA during monocytic differentiation to explore the correlations between the expression levels of miRNAs and mRNA, either negative or positive. After treatment of TPA, U937 cells present functional differentiation markers as well as specific cell morphology, which were confirmed by flow cytometric analysis and Wright-Giemsa staining.Here we use a miRNA microarray (CapitalBio, Beijing, China) containing 509 well-characterized human, mouse and rat miRNAs and various controls to profile the expression levels of miRNA in normal U937 cells and differentiated U937 cells. Three independent biological experiments were performed, and for each differentiated and control sample, two hybridizations were performed by using a reversal fluorescent strategy.

ORGANISM(S): Homo sapiens

SUBMITTER: jing wang 

PROVIDER: E-GEOD-26376 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Microarray profiling of monocytic differentiation reveals miRNA-mRNA intrinsic correlation.

Wang Jing J   Xiang Guangxing G   Mitchelson Keith K   Zhou Yuxiang Y  

Journal of cellular biochemistry 20110901 9

MiRNAs (microRNAs) are small non-coding RNAs involved in mammalian gene expression of cellular processes including differentiation, apoptosis and cancer development. Both specific miRNAs and mRNAs have been identified during monocytic differentiation, but their interactions have not been fully characterized. Here we report that by genome-wide microarray analysis for U937 monocytic differentiation induced by TPA, a large number of miRNAs and mRNAs were differentially expressed, and by bioinformat  ...[more]

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