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Exosome miRNA protein interactions in brown adipocytes

ABSTRACT: We have identified several nucleotide motifs (caug, cgggag=S2) that promote exosome sorting of miRNA in different cell types including brown adipocytes. In order to identify which proteins might recognize and bind to these motifs, we have performed co-precipitations of proteins binding biotinylated forms of miRNAs containing the aforementioned motifs or none - using streptavidin beads incubated with brown adipocytes cell lysates. We have included two types of controls: negative poly-A control and a scramble miRNA.

INSTRUMENT(S): Exploris 480

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Birgit Schilling

PROVIDER: MSV000086780 | MassIVE | Sat Jan 30 17:21:00 GMT 2021

SECONDARY ACCESSION(S): PXD023895

REPOSITORIES: MassIVE

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