Unknown,Transcriptomics,Genomics,Proteomics

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Whole-genome nucleosome mapping in meiotic diploid S. cerevisiae


ABSTRACT: We determined nucleosome positions throughout the genome in diploid S. cerevisiae undergoing early stages of synchronous meiosis. This study sought to assess if systematic reorganization of nucleosomes occurs during meiotic prophase at or near sites of DNA double strand break formation. Six samples were sequenced, each a separate time point from either of two meiotic time courses. Dataset N1 consists of samples collected at 0, 1, 2, and 3 hr in meiosis; Dataset N2 consists of samples collected at 0 and 4 hr. Time courses, sample preparation, and sequencing were carried out in separate laboratories for the two datasets: Hochwagen lab (Whitehead Institute) for Dataset N1; Keeney lab (Howard Hughes Medical Institute; Memorial Sloan-Kettering Cancer Center) for Dataset N2.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Scott keeney 

PROVIDER: E-GEOD-26452 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation.

Pan Jing J   Sasaki Mariko M   Kniewel Ryan R   Murakami Hajime H   Blitzblau Hannah G HG   Tischfield Sam E SE   Zhu Xuan X   Neale Matthew J MJ   Jasin Maria M   Socci Nicholas D ND   Hochwagen Andreas A   Keeney Scott S  

Cell 20110301 5


The nonrandom distribution of meiotic recombination influences patterns of inheritance and genome evolution, but chromosomal features governing this distribution are poorly understood. Formation of the DNA double-strand breaks (DSBs) that initiate recombination results in the accumulation of Spo11 protein covalently bound to small DNA fragments. By sequencing these fragments, we uncover a genome-wide DSB map of unprecedented resolution and sensitivity. We use this map to explore how DSB distribu  ...[more]

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