Proteomics

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Translational repression of the Drosophila nanos mRNA involves the RNA helicase Belle and RNA coating by Me31B and Trailer hitch


ABSTRACT: The maternal mRNA nanos is required for anterior-posterior patterning in the early Drosophila melanogaster development. The nanos-mRNA is translationally repressed and deadenylated during the first two hours of embryogenesis. A stem-loop structure in the 3'-UTR of this RNA (SRE - Smaug recogniiton element) is bound by the Smaug protein which recruits a protein complex to the RNA to sequester its translation and intiate transcript degradation via the ccr4/not-complex. To analyse the composition of this repressor complex, biotinylated RNAs carrying two SRE stem-loops were used for an affinity purification of bound proteins. As a negative control an RNA was used that contained two point mutations in the SRE loop, that abolish binding of Smaug. Seven subunits were identified as components of the repressor complex. The main deadenylase in Drosophila, the ccr4/not-complex was also significantly enriched.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Embryo, Early Embryonic Cell

SUBMITTER: Michael Götze  

LAB HEAD: Elmar Wahle

PROVIDER: PXD006596 | Pride | 2017-07-18

REPOSITORIES: Pride

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151124mqpar.xml Xml
OxidationMSites.txt Txt
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Probe26.raw Raw
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Publications

Translational repression of the <i>Drosophila nanos</i> mRNA involves the RNA helicase Belle and RNA coating by Me31B and Trailer hitch.

Götze Michael M   Dufourt Jérémy J   Ihling Christian C   Rammelt Christiane C   Pierson Stephanie S   Sambrani Nagraj N   Temme Claudia C   Sinz Andrea A   Simonelig Martine M   Wahle Elmar E  

RNA (New York, N.Y.) 20170712 10


Translational repression of maternal mRNAs is an essential regulatory mechanism during early embryonic development. Repression of the <i>Drosophila nanos</i> mRNA, required for the formation of the anterior-posterior body axis, depends on the protein Smaug binding to two Smaug recognition elements (SREs) in the <i>nanos</i> 3' UTR. In a comprehensive mass spectrometric analysis of the SRE-dependent repressor complex, we identified Smaug, Cup, Me31B, Trailer hitch, eIF4E, and PABPC, in agreement  ...[more]

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