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Transcription profiling of human umbilical cord blood and adult bone marrow CD34+CD33-CD38-Rho(lo)c-kit+ cells to identify candidate regulators of HSC self-renewal versus differentiation fate decisions

ABSTRACT: The global gene expression profiles of human umbilical cord blood and adult bone marrow CD34+CD33-CD38-Rho(lo)c-kit+ cells, enriched for hematopoietic stem/progenitor cells (HSC) with CD34+CD33-CD38-Rho(hi) cells, enriched in committed hematopoietic progenitor cells (HPC), were compared to identify candidate regulators of HSC self-renewal versus differentiation fate decisions.

ORGANISM(S): Homo sapiens

SUBMITTER: Craig Eckfeldt

PROVIDER: E-GEOD-2666 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Functional analysis of human hematopoietic stem cell gene expression using zebrafish.

Eckfeldt Craig E CE Mendenhall Eric M EM Flynn Catherine M CM Wang Tzu-Fei TF Pickart Michael A MA Grindle Suzanne M SM Ekker Stephen C SC Verfaillie Catherine M CM

PLoS biology 20050705 8

Although several reports have characterized the hematopoietic stem cell (HSC) transcriptome, the roles of HSC-specific genes in hematopoiesis remain elusive. To identify candidate regulators of HSC fate decisions, we compared the transcriptome of human umbilical cord blood and bone marrow (CD34+)(CD33-)(CD38-)Rho(lo)(c-kit+) cells, enriched for hematopoietic stem/progenitor cells with (CD34+)(CD33-)(CD38-)Rho(hi) cells, enriched in committed progenitors. We identified 277 differentially expresse ...[more]

PMID: 16089502

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