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Vaginal epithelial gene expression in postmenopausal women suffering from vaginal dryness

ABSTRACT: The onset of menopause is accompanied by a dramatic increase in reported symptoms of vaginal dryness, soreness, irritation or itching, pain with intercourse and bleeding after intercourse. Collectively these affect 25-50% of women of post-menopausal age and significantly impact their quality of life. To examine how gene expression differs between these groups, surface vaginal epithelial cells were collected from postmenopausal women suffering from vaginal dryness and appropriate controls not suffering from dryness. Affymetrix GeneChip Human 1.0 ST microarrays were performed on RNA isolated from ten participants. Suitable RNA was extracted from ten participants which were classified into two groups, the dryness and control groups, based on diagnosis of dryness by a nurse during gynecoligical examination.

ORGANISM(S): Homo sapiens

SUBMITTER: Jordan Bisanz

PROVIDER: E-GEOD-26761 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Vaginal microbiome and epithelial gene array in post-menopausal women with moderate to severe dryness.

Hummelen Ruben R Macklaim Jean M JM Bisanz Jordan E JE Hammond Jo-Anne JA McMillan Amy A Vongsa Rebecca R Koenig David D Gloor Gregory B GB Reid Gregor G

PloS one 20111102 11

After menopause, many women experience vaginal dryness and atrophy of tissue, often attributed to the loss of estrogen. An understudied aspect of vaginal health in women who experience dryness due to atrophy is the role of the resident microbes. It is known that the microbiota has an important role in healthy vaginal homeostasis, including maintaining the pH balance and excluding pathogens. The objectives of this study were twofold: first to identify the microbiome of post-menopausal women with ...[more]

PMID: 22073175

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Project description:The onset of menopause is accompanied by a dramatic increase in reported symptoms of vaginal dryness, soreness, irritation or itching, pain with intercourse and bleeding after intercourse. Collectively these affect 25-50% of women of post-menopausal age and significantly impact their quality of life. To examine how gene expression differs between these groups, surface vaginal epithelial cells were collected from postmenopausal women suffering from vaginal dryness and appropriate controls not suffering from dryness. Affymetrix GeneChip Human 1.0 ST microarrays were performed on RNA isolated from ten participants.

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