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Expression data from human fibroblasts 6 and 12 hours after 8-oxoguanine treatment

ABSTRACT: 8-oxoguanine is excised from DNA by 8-oxoguanine DNA glycosylase-1 during DNA base excision repair. We used microarrays to gain insight into the potential biological function of the excised, free 8-oxoguanine base. MRC5, human diploid fibroblasts were exposed to 8-oxoguanine and changes in gene expression compared to mock control were analyzed 6 hours after exposure. MRC5, human diploid fibroblasts were exposed to 8-oxoguanine and changes in gene expression compared to mock control were analyzed 12 hours after exposure.

ORGANISM(S): Homo sapiens

SUBMITTER: Istvan Boldogh

PROVIDER: E-GEOD-26813 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Activation of ras signaling pathway by 8-oxoguanine DNA glycosylase bound to its excision product, 8-oxoguanine.

Boldogh Istvan I Hajas Gyorgy G Aguilera-Aguirre Leopoldo L Hegde Muralidhar L ML Radak Zsolt Z Bacsi Attila A Sur Sanjiv S Hazra Tapas K TK Mitra Sankar S

The Journal of biological chemistry 20120508 25

8-Oxo-7,8-dihydroguanine (8-oxoG), arguably the most abundant base lesion induced in mammalian genomes by reactive oxygen species, is repaired via the base excision repair pathway that is initiated with the excision of 8-oxoG by OGG1. Here we show that OGG1 binds the 8-oxoG base with high affinity and that the complex then interacts with canonical Ras family GTPases to catalyze replacement of GDP with GTP, thus serving as a guanine nuclear exchange factor. OGG1-mediated activation of Ras leads t ...[more]

PMID: 22568941

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Expression data from human fibroblasts 6 and 12 hours after 8-oxoguanine treatment

Project description:8-oxoguanine is excised from DNA by 8-oxoguanine DNA glycosylase-1 during DNA base excision repair. We used microarrays to gain insight into the potential biological function of the excised, free 8-oxoguanine base.

2011-01-27 | GSE26813 | GEO

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2007-12-01 | E-TABM-382 | biostudies-arrayexpress

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OSLN Reprogramming cells: empty vector vs. HIF2M-NM-1 over-expression

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Real-time quantitative PCR analysis of mouse lungs

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