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Time kinetics of gene expression in NK92 cells after Plasmodium falciparum-iRBC encounter

ABSTRACT: To study the effect of Plasmodium falciparum-infected erythrocytes on gene expression in NK92 cells, microarray analysis after 6, 12 and 24 hours of co-culture with either uRBC or iRBC was performed. The aim was to identify pathways in NK92 cells that are switched on after iRBC encounter in a time-dependent manner that will help to understand the mechanisms in innate immune defenses against Plasmodium falciparum infection. Variation in gene expression of NK92 cells was determined after 6, 12, and 24 hours of co-culture with either infected or uninfected RBC compared to timepoint 0 (start of co-culture, untreated control). All experiments were done in triplicate, that means that samples were collected in 3 different experiments (A, B, and C) each time after 0, 6, 12 and 24 hours of co-culture.

ORGANISM(S): Homo sapiens

SUBMITTER: Evelyn Boettger

PROVIDER: E-GEOD-26876 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Limited response of NK92 cells to Plasmodium falciparum-infected erythrocytes.

de Carvalho Elisandra Grangeiro EG Böttger Evelyn E Hoang Van Tong VT Kremsner Peter G PG Kun Jürgen F J JF

Malaria journal 20111021

<h4>Background</h4>Mechanisms by which anti-malarial immune responses occur are still not fully clear. Natural killer (NK) cells are thought to play a pivotal role in innate responses against Plasmodium falciparum. In this study, the suitability of NK92 cells as models for the NK mechanisms involved in the immune response against malaria was investigated.<h4>Methods</h4>NK92 cells were assessed for several signs of activation and cytotoxicity due to contact to parasites and were as well examined ...[more]

PMID: 22018162

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