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Drosophila LID RNAi gene expression profiling

ABSTRACT: H3K4me3 is a histone modification related to gene activation. LID is a demethylase acting on this residue and therefore, it could be important for proper expression of genes in Drosophila developing tissues, such as wing imaginal discs We used microarrays to analyse the changes in gene expression after lid depletion by RNAi, both in a wild type background and in a mutant background Two replicates were obtained on Nov 2010 for wild type white drosophila, GFP RNAi control and LID RNAi (on a LID mutant background).

ORGANISM(S): Drosophila melanogaster

SUBMITTER: David Rossell 

PROVIDER: E-GEOD-26895 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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dKDM5/LID regulates H3K4me3 dynamics at the transcription-start site (TSS) of actively transcribed developmental genes.

Lloret-Llinares Marta M   Pérez-Lluch Sílvia S   Rossell David D   Morán Tomás T   Ponsa-Cobas Joan J   Auer Herbert H   Corominas Montserrat M   Azorín Fernando F  

Nucleic acids research 20120816 19

H3K4me3 is a histone modification that accumulates at the transcription-start site (TSS) of active genes and is known to be important for transcription activation. The way in which H3K4me3 is regulated at TSS and the actual molecular basis of its contribution to transcription remain largely unanswered. To address these questions, we have analyzed the contribution of dKDM5/LID, the main H3K4me3 demethylase in Drosophila, to the regulation of the pattern of H3K4me3. ChIP-seq results show that, at  ...[more]

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