Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression profiling of mammary stem cell enriched fractions and other fractions from fetal and adult mouse.

ABSTRACT: Examination of genome-wide, gene expression level differences among adult and fetal mammary sub-populations including fetal CD24high cells and adult CD49fhighCD24medium cells which harbor enriched mammary stem cell activity. A genome wide 12-plex expression array using linear-amplified (Eberwine) mRNA from dissociated and FACS sorted fetal and adult mammary gland populations. Samples represent independent pooled biological replicates.

ORGANISM(S): Mus musculus

SUBMITTER: Benjamin Spike

PROVIDER: E-GEOD-27027 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A mammary stem cell population identified and characterized in late embryogenesis reveals similarities to human breast cancer.

Spike Benjamin T BT Engle Dannielle D DD Lin Jennifer C JC Cheung Samantha K SK La Justin J Wahl Geoffrey M GM

Cell stem cell 20120201 2

Gene expression signatures relating mammary stem cell populations to breast cancers have focused on adult tissue. Here, we identify, isolate, and characterize the fetal mammary stem cell (fMaSC) state since the invasive and proliferative processes of mammogenesis resemble phases of cancer progression. fMaSC frequency peaks late in embryogenesis, enabling more extensive stem cell purification than achieved with adult tissue. fMaSCs are self-renewing, multipotent, and coexpress multiple mammary li ...[more]

PMID: 22305568

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Project description:Glioblastoma multiforme (GBM) is the most prevalent type of adult brain tumor, and one of the deadliest tumors known to mankind. The genetic understanding of GBM is, however, limited, and the molecular mechanisms which facilitate GBM cell survival and growth within the tumor microenvironment are largely unknown. We applied digital karyotyping and single nucleotide polymorphism (SNP) arrays to screen for copy number changes in GBM samples and found that the most frequently amplified region is at chromosome 7p11.2. The high resolution of digital karyotyping and SNP arrays permits the precise delineation of amplicon boundaries and has enabled identification of the minimal region of amplification at 7p11.2, which contains two genes, EGFR and SEC61?. SEC61? encodes a subunit of a heterotrimeric protein channel located in the endoplasmic reticulum (ER). In addition to its high frequency of gene amplification in GBMs, SEC61? is also remarkably overexpressed in 77% of GBMs, but not in lower-grade gliomas. The siRNA-mediated knockdown of SEC61? expression in tumor cells led to growth suppression and apoptosis. Furthermore, we showed that pharmacological ER stress agents induce SEC61? expression in GBM cells. Together, these results indicate that aberrant expression of SEC61? serves significant roles in GBM cell survival, likely via a mechanism that is involved in the cytoprotective ER stress-adaptive response to the tumor microenvironment. hSETD1A was silenced in HCT116 cells using retrovirus harboring shRNA specifically against the hSETD1A gene. 10?g RNA was reverse transcribed to cDNA using the Applied Biosystems High Capacity cDNA Reverse Transcription kit according to the manufacturer’s instructions (Applied Biosystems). cDNA products were treated with 100ng RNaseA and then purified using the Qiagen PCR purification kit according to the manufacturer’s instructions. NimbleGen Human Gene Expression array was purchased from Roche Applied Sciences. cDNA samples were labeled, Cy3 hybridized, and processed at the FSU NimbleGen Microarray Facility at Florida State University.

Dataset Information

Expression profiling of mammary stem cell enriched fractions and other fractions from fetal and adult mouse.

Publications

A mammary stem cell population identified and characterized in late embryogenesis reveals similarities to human breast cancer.

Similar Datasets

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