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Expression analysis of zebrafish liver after exposure in naphthalene

ABSTRACT: Investigation of whole genome gene expression level changes in zebrafish liver under naphthalene treatedï¼?high-concentration group and low-concentration groupï¼?, compared to the solvent control group revealed that 76 genes and 88 genes were differentially expressed respectively in the fish caged at the low-concentration and high-concentration. KEGG pathway and GO analysis of the differentially expressed genes, showed significant enrichment in several meaningful categories. Healthy 5-month-old adult zebrafish (AB strain) maintenance and chemical exposure to 84Î¼g/L and 840Î¼g/L naphthalene and 0.005% Dimethyl sulfoxide as the solvent control were performed according to published research protocols. Prior to exposure, the fish were acclimatized in 50L aerated fresh water in glass tanks for 2 weeks, under controlled environmental conditions with the water temperature maintained at 26Â±0.5â?? for 16-h light and 8-h dark photoperiod. After 21 d of treatment, adult zebrafish livers were removed by dissection, and immediately transferred to RNA-later Stabilization Reagent(Qiagen,76106) , prior to storage at 4â?? for histopathological and microarray analysis. NimbleGen Gene Expression 12X135K zebrafish microarrays and One-Color DNA labeling Kit (NimbleGen, WI) were used for genome-wide expression analysis of naphthalene-treated zebrafish.

ORGANISM(S): Danio rerio

SUBMITTER: Hongshan Chen

PROVIDER: E-GEOD-74776 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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