Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression levels in strained vs. non-strained Calu-3 lung epithelial cells

ABSTRACT: Ventilator induced lung injury can lead to serious conditions like ARDS which are associated with a high mortality (around 30%, Stapleton et al., Chest, 2005). We hypothesized that changes of expression levels of different genes would lead us to the identification of critical target genes, which might influence the inflammation and outcome associated with this condition. We used human whole genome U133 Plus 2.0 microarrays to detail the changes of gene expression and identified distinct classes of up-regulated genes during this process. Confluent non strained and strained human Calu-3 cells were selected for RNA extraction and hybridization on Affymetrix microarrays. The experiment was performed with 3 replicates.

ORGANISM(S): Homo sapiens

SUBMITTER: Holger Eltzschig

PROVIDER: E-GEOD-27128 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

HIF1A reduces acute lung injury by optimizing carbohydrate metabolism in the alveolar epithelium.

Eckle Tobias T Brodsky Kelley K Bonney Megan M Packard Thomas T Han Jun J Borchers Christoph H CH Mariani Thomas J TJ Kominsky Douglas J DJ Mittelbronn Michel M Eltzschig Holger K HK

PLoS biology 20130924 9

<h4>Background</h4>While acute lung injury (ALI) contributes significantly to critical illness, it resolves spontaneously in many instances. The majority of patients experiencing ALI require mechanical ventilation. Therefore, we hypothesized that mechanical ventilation and concomitant stretch-exposure of pulmonary epithelia could activate endogenous pathways important in lung protection.<h4>Methods and findings</h4>To examine transcriptional responses during ALI, we exposed pulmonary epithelia t ...[more]

PMID: 24086109

Publication: 1/2

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Project description:Background: Basal cells within the human airway epithelium constitute the stem/progenitor cells for other epithelial cell types. Basal cells respond to mucosal injury and damage to the airway mucosa in an ordered sequence of spreading, migration, proliferation and phenotype shifting (differentiation) to other needed cell types. However, dynamic gene transcription in the early events of injury and repair has not been examined in these cells. Methodology and findings: Airway epithelial cells were obtained from donated lungs and grown in submersion culture on pliable membranes to obtain a pure population of basal cells. Microarrays were used to assess the transcriptome of basal cells 8 and 24 hr after mechanical injury (MI), or to cyclic stretch (CS) in a Flexcell system (0.5 Hz, 20% distension), or both treatments. We identified 121 signature genes with > 2-fold higher differential expression (DE) 8 hr after MI; expression of nearly all of these genes returned to baseline at 24 hr after injury. In cells subjected to CS, little change in DE was noted at 8 hr, whereas at 24 hr a CS signature of 1430 DE genes were identified. The MI signature was characterized by genes encoding growth factor receptors related to the EGF pathway, IL-6, IL-8 and IL-33, extracellular matrix components, and NF-kB and p38-MAPK signaling pathways, whereas the CS signature was characterized by a broad range of genes that did not identify specific signaling pathways. Combined MI and CS at 8 hr elicited DE of down-regulated genes not seen with either stimulus alone, and at 24 hr elicited DE that was similar to that seen with CS alone. Conclusion and significance: The human airway basal epithelial cell transcription signature in the first hours after MI, after CS, and after both stimuli identifies unique differentially expressed genes and pathways that may be important in the early molecular response and biology to airway injury. Total RNA obtained from primary (AEC) and differentiated (dAEC) human airway epithelial cells subjected to 8 or 24 hours in vitro mechanical or cyclic stretch or both injuries compared to sham control as well as to type of injury. Cells were collected from four donated lungs and cultured separated in submission or air liquid interface condition prior to injury for various durations.

Dataset Information

Expression levels in strained vs. non-strained Calu-3 lung epithelial cells

Publications

HIF1A reduces acute lung injury by optimizing carbohydrate metabolism in the alveolar epithelium.

Similar Datasets

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