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Strand specific sequencing illustrates the complex transcriptional response of Legionella pneumophila during infection

ABSTRACT: Legionella pneumophila is a Gram-negative facultative intracellular human pathogen with a distinct biphasic lifestyle. One of its primary environmental hosts in the free-living amoeba Acanthamoeba castellanii and its infection by L. pneumophila mimics that seen in human macrophages. Here we present analysis of strand specific sequencing of the transcriptional response of L. pneumophila in broth growth and in infection of A. castellanii. Examination of 2 infection and 3 broth growth time points

ORGANISM(S): Legionella pneumophila

SUBMITTER: James Prendergast

PROVIDER: E-GEOD-27232 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Sequencing illustrates the transcriptional response of Legionella pneumophila during infection and identifies seventy novel small non-coding RNAs.

Weissenmayer Barbara A BA Prendergast James G D JG Lohan Amanda J AJ Loftus Brendan J BJ

PloS one 20110303 3

Second generation sequencing has prompted a number of groups to re-interrogate the transcriptomes of several bacterial and archaeal species. One of the central findings has been the identification of complex networks of small non-coding RNAs that play central roles in transcriptional regulation in all growth conditions and for the pathogen's interaction with and survival within host cells. Legionella pneumophila is a gram-negative facultative intracellular human pathogen with a distinct biphasic ...[more]

PMID: 21408607

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