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Fish oil/pectin temporally alters gene expression profiles in exfoliated rat colonocytes throughout oncogenesis


ABSTRACT: We have demonstrated that fish oil/pectin (FO/P) diets protect against colon cancer compared to corn oil/cellulose (CO/C) by upregulating apoptosis and suppressing proliferation. To elucidate the mechanisms whereby FO/P diets induce apoptosis and suppress proliferation during the tumorigenic process, we analyzed the temporal gene expression profiles from exfoliated rat colonocytes. KEYWORDS: Fish oil/pectin, pectin, exfoliated colonocytes, gene expression, apoptosis, colon cancer, chemoprevention Rats consumed diets containing FO/P or CO/C and were injected with azoxymethane (AOM, 2x, 15 mg/kg BW, s.c.). Feces collected at the initiation, aberrant crypt foci (ACF), and tumor stages of colon cancer (24 h (n=20), 7 wk (n=37), and 28 wk (n=28) after AOM injection, respectively) was used for poly A(+) RNA extraction. Gene expression signatures were determined using Codelink arrays.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Youngmi Cho 

PROVIDER: E-GEOD-27266 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A chemoprotective fish oil- and pectin-containing diet temporally alters gene expression profiles in exfoliated rat colonocytes throughout oncogenesis.

Cho Youngmi Y   Kim Hyemee H   Turner Nancy D ND   Mann John C JC   Wei Jiawei J   Taddeo Stella S SS   Davidson Laurie A LA   Wang Naisyin N   Vannucci Marina M   Carroll Raymond J RJ   Chapkin Robert S RS   Lupton Joanne R JR  

The Journal of nutrition 20110420 6


We have demonstrated that fish oil- and pectin-containing (FO/P) diets protect against colon cancer compared with corn oil and cellulose (CO/C) by upregulating apoptosis and suppressing proliferation. To elucidate the mechanisms whereby FO/P diets induce apoptosis and suppress proliferation during the tumorigenic process, we analyzed the temporal gene expression profiles from exfoliated rat colonocytes. Rats consumed diets containing FO/P or CO/C and were injected with azoxymethane (AOM; 2 times  ...[more]

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