Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome and translational signaling following endurance exercise in trained skeletal muscle: impact of dietary protein


ABSTRACT: Impact of protein ingestion following 1 h intense cycling on the induced transcriptome and signaling in biopsy samples from endurance-trained men, relative to isocaloric control Single blind, randomized, crossover design comprising two experimental blocks. During the blocks, exercise and diet were controlled and the intervention consisted of the ingestion of a protein-enriched and control beverage, with outcome measures obtained from skeletal muscle tissue collected following a bout of intense cycling. Total RNA were obtained from quadriceps. Each of the two serves were isocaloric and provided 0.2 g/kg fat (freeze dried canola oil) with either 1.2 g/kg carbohydrate (1:1 maltodextrin:fructose) and 0.4 g/kg protein (2:1 milk protein concentrate:whey isolate); or 1.6 g/kg carbohydrate (1:1 maltodextrin:fructose).

ORGANISM(S): Homo sapiens

SUBMITTER: Frederic Raymond 

PROVIDER: E-GEOD-27285 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptome and translational signaling following endurance exercise in trained skeletal muscle: impact of dietary protein.

Rowlands David S DS   Thomson Jasmine S JS   Timmons Brian W BW   Raymond Frédéric F   Fuerholz Andreas A   Mansourian Robert R   Zwahlen Marie-Camille MC   Métairon Sylviane S   Glover Elisa E   Stellingwerff Trent T   Kussmann Martin M   Tarnopolsky Mark A MA  

Physiological genomics 20110705 17


Postexercise protein feeding regulates the skeletal muscle adaptive response to endurance exercise, but the transcriptome guiding these adaptations in well-trained human skeletal muscle is uncharacterized. In a crossover design, eight cyclists ingested beverages containing protein, carbohydrate and fat (PTN: 0.4, 1.2, 0.2 g/kg, respectively) or isocaloric carbohydrate and fat (CON: 1.6, 0.2 g/kg) at 0 and 1 h following 100 min of cycling. Biopsies of the vastus lateralis were collected at 3 and  ...[more]

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