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The role of FOG-1 in GATA-1 transcriptional regulation of megakaryocyte differentiation

ABSTRACT: We explored the role of FOG-1 in GATA-1 transcriptional regulation of megakaryocyte differentiation through expression of wild-type GATA-1 and the FOG-binding mutant of GATA-1 (GATA-1^V205G) in G1ME cells. G1ME cells were derived from Gata1-null mouse ES cells and have both megakaryocyte and erythrocyte differentiation potential upon reconstitution of GATA-1 expression (Stachura 2006). HA-tagged wild-type or mutant GATA-1 were expressed in G1ME cells grown in TPO via retroviral transductions. The cells were sorted for GFP positivity 68 hours post-transduction and then were allowed to recover in normal growth medium for 4h. Total RNA was then isolated using RNeasy kit from Qiagen 72 hours post-transduction.

ORGANISM(S): Mus musculus

SUBMITTER: Timothy Chlon

PROVIDER: E-GEOD-35695 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Chromatin occupancy analysis reveals genome-wide GATA factor switching during hematopoiesis.

Doré Louis C LC Chlon Timothy M TM Brown Christopher D CD White Kevin P KP Crispino John D JD

Blood 20120301 16

There are many examples of transcription factor families whose members control gene expression profiles of diverse cell types. However, the mechanism by which closely related factors occupy distinct regulatory elements and impart lineage specificity is largely undefined. Here we demonstrate on a genome wide scale that the hematopoietic GATA factors GATA-1 and GATA-2 bind overlapping sets of genes, often at distinct sites, as a means to differentially regulate target gene expression and to regula ...[more]

PMID: 22383799

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The role of FOG-1 in GATA-1 transcriptional regulation of megakaryocyte differentiation

Project description:We explored the role of FOG-1 in GATA-1 transcriptional regulation of megakaryocyte differentiation through expression of wild-type GATA-1 and the FOG-binding mutant of GATA-1 (GATA-1^V205G) in G1ME cells.

2012-03-05 | GSE35695 | GEO

Cofactor-mediated Restriction of GATA-1 Chromatin Occupancy Coordinates Lineage-specific Gene Expression

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2012-07-29 | E-GEOD-35708 | biostudies-arrayexpress

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Genome-wide analysis of the role of FOG-1 in GATA-1 chromatin occupancy

Project description:We report ChIP-Seq data for GATA-1 and the FOG-binding mutant of GATA-1 (GATA-1^V205G) in G1ME cells, a Gata1-null cell line with both erythroid and megakaryocytic differentiation potential. We introduced HA-tagged GATA-1 or V205G into G1ME cells via retroviral transduction. The cells were crosslinked at 48h post-transduction, and an HA antibody was used for chromatin immunoprecipitation (ChIP). ChIP and input samples were sequenced on Illumina GAII high-throughput sequencer. The data reveal GATA-1-specific and V205G-specific bidning sites, indicating that FOG-1 both faacilitates and prohibits GATA-1 chromatin occupancy in a context-dependent manner. Examinaton of chromatin occupancy of GATA-1 anda FOG-binding mutant of GATA-1 in G1ME cells cultured in TPO.

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2009-06-30 | GSE6808 | GEO

Expression profile of hemin induced erythroid differentiation, over-expressing and knocking-down GATA-1, EKLF and NF-E2 in K562s

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2011-12-31 | E-GEOD-30380 | biostudies-arrayexpress

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