Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of mouse mutants DBA/2JNIA primary myoblasts at 8 and 23 months to investigate aging

ABSTRACT: Comparison of primary myoblasts isolated from 8- and 23-month-old DBA/2JNIA mice.

ORGANISM(S): Mus musculus

SUBMITTER: Marjorie Beggs

PROVIDER: E-GEOD-273 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Alterations in the TGFbeta signaling pathway in myogenic progenitors with age.

Beggs Marjorie L ML Nagarajan Radhakrishnan R Taylor-Jones Jane M JM Nolen Greg G Macnicol Melanie M Peterson Charlotte A CA

Aging cell 20041201 6

Myogenic progenitors in adult muscle are necessary for the repair, maintenance and hypertrophy of post-mitotic muscle fibers. With age, fat deposition and fibrosis contribute to the decline in the integrity and functional capacity of muscles. In a previous study we reported increased accumulation of lipid in myogenic progenitors obtained from aged mice, accompanied by an up-regulation of genes involved in adipogenic differentiation. The present study was designed to extend our understanding of h ...[more]

PMID: 15569352

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Project description:Purpose: The DBA/2J mouse is a model for secondary angle-closure glaucoma due to iris atrophy and pigment dispersion, which ultimately leads to increased intraocular pressure (IOP). We sought to correlate changes in retinal gene expression with glaucoma-like pathology by performing microarray analysis of retinal RNA from DBA/2J mice at 3 months before disease onset, and at 8 months, after IOP elevation. Methods: IOP was monitored monthly in DBA/2J animals by Tono-Pen and animals with normal (3 months) or elevated IOP (8 months) were identified. RNA was prepared from 3 individual retinas at each age, and the RNA was amplified and used to generate biotin-labeled probe for high density mouse Affymetrix arrays (U430.2). A subset of genes was selected for confirmation by quantitative RT-PCR using independent retina samples from DBA/2J animals at 3, 5 and 8 months of age, and compared to retinas from C57BL/6J control animals at 3 and 8 months. Results: There were changes in expression of 68 genes, with 32 genes increasing and 36 genes decreasing at 8 months versus 3 months. Upregulated genes were associated with immune response, glial activation, signaling and gene expression, while down-regulated genes included multiple crystallin genes. Significant changes in 9 upregulated genes and 2 downregulated genes were confirmed by quantitative RT-PCR, with some showing changes in expression by 5 months. Conclusions: DBA/2J retina shows evidence for glial activation and an immune-related response following IOP elevation, similar to what has been reported following acute elevation of IOP in other models. IOP was monitored monthly in DBA/2J animals by Tono-Pen and animals with normal (3 months) or elevated IOP (8 months) were identified. RNA was prepared from 3 individual retinas at each age, and the RNA was amplified and used to generate biotin-labeled probe for high density mouse Affymetrix arrays (U430.2). A subset of genes was selected for confirmation by quantitative RT-PCR using independent retina samples from DBA/2J animals at 3, 5 and 8 months of age, and compared to retinas from C57BL/6J control animals at 3 and 8 months.

Dataset Information

Transcription profiling of mouse mutants DBA/2JNIA primary myoblasts at 8 and 23 months to investigate aging

Publications

Alterations in the TGFbeta signaling pathway in myogenic progenitors with age.

Similar Datasets

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