Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse aging lung from two strains C57BL/6J and DBA/2J


ABSTRACT: We hypothesize that gene expression in the aging lungs of these two strains of mice are divergent thus contributing to the disparity in the phenotypes.re specifically, (1) Aging DBA/2J mice compared to aging C57BL/6 mice are known to be accelerated in their lung physiology andrphometry; (2) C57BL/6J are known to have longer natural longevity than DBA/2J mice. In order to test these hypotheses at the gene expression level, we utilized microarray analysis to examine transcriptional differences between aging lungs of both strains of mice. Experiment Overall Design: This study utilizes microarray analysis to test these hypotheses. Three sets of lungs were harvested from both strains at each time point (C57BL/6J: 2, 18, AND 26s; DBA/2J: 2 and 18s). RNA was isolated and used for global gene expression profiling (Affymetrixuse 430 2.0 array). Statistically significant gene expression was determined as a minimum 6 counts of 9 pairwise comparisons, minimum 1.5-fold change, and p < 0.05. Further, Absolute | FC - FC SEM | >= 1.5.

ORGANISM(S): Mus musculus

SUBMITTER: vikas misra 

PROVIDER: E-GEOD-6591 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global expression profiles from C57BL/6J and DBA/2J mouse lungs to determine aging-related genes.

Misra Vikas V   Lee Hannah H   Singh Anju A   Huang Kewu K   Thimmulappa Rajesh K RK   Mitzner Wayne W   Biswal Shyam S   Tankersley Clarke G CG  

Physiological genomics 20070828 3


This study identified gene expression profiles that provided evidence for genomic mechanisms underlying the pathophysiology of aging lung. Aging lungs from C57BL/6 (B6) and DBA/2 (D2) mouse strains differ in physiology and morphometry. Lungs were harvested from B6 mice at 2, 18, and 26 mo and from D2 mice at 2 and 18 mo of age. Purified RNA was subjected to oligonucleotide microarray analyses, and differential expression analyses were performed for comparison of various data sets. A significant  ...[more]

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