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Genome-wide analysis of gene expression perturbed by Pontin shRNA in MCF7 subjected to normoxic and hypoxic conditions

ABSTRACT: In order to investigate the role of Pontin methylation on the expression of hypoxia responsive genes across the whole genome, we performed a microarray analysis from RNAs isolated from MCF7 cells expressing either control shRNA (shNS) or Pontin shRNA(shPontin) in normoxic and hypoxic conditions. MCF7 cells were transiently transfected with either non-specific shRNA or Pontin shRNA in replicates. Cells were grown for 24 hours before being exposed to either normoxic (21% Oxygen) or hypoxic condition (1% oxygen) for 6,9 hours. Cells were then lysed and RNA isolated.

ORGANISM(S): Homo sapiens

SUBMITTER: Bhin Jin-hyuk

PROVIDER: E-GEOD-27813 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Hypoxia-induced methylation of a pontin chromatin remodeling factor.

Lee Jason S JS Kim Yunho Y Bhin Jinhyuk J Shin Hi-Jai R HJ Nam Hye Jin HJ Lee Seung Hoon SH Yoon Jong-Bok JB Binda Olivier O Gozani Or O Hwang Daehee D Baek Sung Hee SH

Proceedings of the National Academy of Sciences of the United States of America 20110808 33

Pontin is a chromatin remodeling factor that possesses both ATPase and DNA helicase activities. Although Pontin is frequently overexpressed in human cancers of various types and implicated in oncogenic functions, the upstream signaling network leading to the regulation of Pontin that in turn affects transcription of downstream target genes has not been extensively studied. Here, we identify Pontin is methylated by G9a/GLP methyltransferases in hypoxic condition and potentiates HIF-1α-mediated ac ...[more]

PMID: 21825155

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