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Expression data from C. elegans (wild type vs. tag-24)

ABSTRACT: To provide insights into the mechanism underlying the enhanced immunity of tag-24/octr-1 animals, we used genome microarrays to find clusters of genes commonly misregulated in tag-24 relative to wild-type animals grown on live P. aeruginosa. Gravid adult wild-type and tag-24/octr-1 animals were lysed using a solution of sodium hydroxide and bleach (ratio of 5:2), washed and the eggs were synchronized for 22 hours in S basal liquid medium at room temperature. Synchronized L1 larval animals were placed onto NGM plates seeded with E. coli OP50 and grown at 20°C until the animals had reached the L4 larval stage. Animals were collected and washed with M9 buffer before transferring to NGM plates containing P. aeruginosa PA14 for 4 hours at 25°C. After 4 hours, animals were collected and washed with M9 buffer, and RNA was extracted using TRIzol reagent (Invitrogen). Residual genomic DNA was removed by DNase treatment (Ambion Austin, TX).

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Alejandro Aballay

PROVIDER: E-GEOD-27867 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Neuronal GPCR controls innate immunity by regulating noncanonical unfolded protein response genes.

Sun Jingru J Singh Varsha V Kajino-Sakamoto Rie R Aballay Alejandro A

Science (New York, N.Y.) 20110407 6030

The unfolded protein response (UPR), which is activated when unfolded or misfolded proteins accumulate in the endoplasmic reticulum, has been implicated in the normal physiology of immune defense and in several human diseases, including diabetes, cancer, neurodegenerative disease, and inflammatory disease. In this study, we found that the nervous system controlled the activity of a noncanonical UPR pathway required for innate immunity in Caenorhabditis elegans. OCTR-1, a putative octopamine G pr ...[more]

PMID: 21474712

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