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Expression data of Medicago truncatula skl1-1 roots treated with S. meliloti wild-type or auxin transport inhibitors

ABSTRACT: Rhizobium and allied bacteria form symbiotic nitrogen-fixing nodules on legume roots. Plant hormones appear to play a role in nodule formation. We treated Medicago truncatula roots with auxin transport inhibitors (ATIs) N-(1-naphthyl)phthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA) to induce the formation of pseudonodules. We compared the transcriptional responses of M. truncatula roots treated with ATIs to roots inoculated with Sinorhizobium meliloti. The transcriptional response of M. truncatula roots 1 and 7 days after ATI treatment were opposite to roots treated with S. meliloti. Three independent biological replicates were performed for each treatment (buffer, ATI and wild-type bacteria) at 21 days after treatment.

ORGANISM(S): Medicago truncatula

SUBMITTER: Adriana Rightmyer

PROVIDER: E-GEOD-28172 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Nitrogen fixation mutants of Medicago truncatula fail to support plant and bacterial symbiotic gene expression.

Starker Colby G CG Parra-Colmenares Adriana L AL Smith Lucinda L Mitra Raka M RM Long Sharon R SR

Plant physiology 20060111 2

The Rhizobium-legume symbiosis culminates in the exchange of nutrients in the root nodule. Bacteria within the nodule reduce molecular nitrogen for plant use and plants provide bacteria with carbon-containing compounds. Following the initial signaling events that lead to plant infection, little is known about the plant requirements for establishment and maintenance of the symbiosis. We screened 44,000 M2 plants from fast neutron-irradiated Medicago truncatula seeds and isolated eight independent ...[more]

PMID: 16407449

Publication: 1/2

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