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Genes differentially expressed in wild-type Medicago truncatula plants during nodulation


ABSTRACT: We used an Affymetrix oligonucleotide microarray consisting of 9,935 tentative consensus (TC)sequences, which are based on cDNA libraries (Mitra et al., 2004 [PMID:15220482]). We examined gene expression of wild-type M. truncatula plants after inoculation with wild-type S. meliloti at 1 d, 4 d, 7d, 14 d, and 21 d. We chose these time points because they span the range of development of the Rhizobium-legume symbiosis, from initiation of the interaction through nitrogen fixation. The gene expression data for the 1-day time point (Mitra et al., 2004 [PMID 15220482]) and the other time points was previously published (Starker el al., 2006 [PMID 16407449]). Three or five independent biological replicates were performed at each time point (1, 4, 7, 14 and 21 days after treatment) and each treatment (buffer and wild-type bacteria).

ORGANISM(S): Medicago truncatula

SUBMITTER: Adriana Rightmyer 

PROVIDER: E-GEOD-28173 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Nitrogen fixation mutants of Medicago truncatula fail to support plant and bacterial symbiotic gene expression.

Starker Colby G CG   Parra-Colmenares Adriana L AL   Smith Lucinda L   Mitra Raka M RM   Long Sharon R SR  

Plant physiology 20060111 2


The Rhizobium-legume symbiosis culminates in the exchange of nutrients in the root nodule. Bacteria within the nodule reduce molecular nitrogen for plant use and plants provide bacteria with carbon-containing compounds. Following the initial signaling events that lead to plant infection, little is known about the plant requirements for establishment and maintenance of the symbiosis. We screened 44,000 M2 plants from fast neutron-irradiated Medicago truncatula seeds and isolated eight independent  ...[more]

Publication: 1/2

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