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Gene Expression Analysis of Transgenic Myc-Driven Liver Tumor Formation and Regression

ABSTRACT: Gene expression changes were examined in transgenic MYC-driven liver cancers at different time points as tumors formed and upon early regression. Time points evaluated include: Control (non-tumor bearing), Pre-tumor (mice were removed from doxyclycine in their diet to induce MYC oncogene expression for 4-5 weeks), Tumor (tumor nodules from mice that had been off of doxycycline for 8-9 weeks) and Early tumor regression (tumor-bearing mice were placed back on doxycycline for 72 hrs to inhibit MYC oncogene expresion). MYC-Driven Mouse Tumor Models are described in Schahaf, et al., Nature, 2004 and Goga, et al., Nature Medicine, 2007. 11 Total Samples: 3 Control, 4 Pre-Tumor, 4 Tumor, 4 Early Tumor Regression. Control = LAP-tTA transgenic mice. Others = TRE-MYC x LAP-tTA double transgenic mice (doxy off).

ORGANISM(S): Mus musculus

SUBMITTER: Andrei Goga

PROVIDER: E-GEOD-28198 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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13C-pyruvate imaging reveals alterations in glycolysis that precede c-Myc-induced tumor formation and regression.

Hu Simon S Balakrishnan Asha A Bok Robert A RA Anderton Brittany B Larson Peder E Z PE Nelson Sarah J SJ Kurhanewicz John J Vigneron Daniel B DB Goga Andrei A

Cell metabolism 20110701 1

Tumor cells have an altered metabolic phenotype characterized by increased glycolysis and diminished oxidative phosphorylation. Despite the suspected importance of glycolysis in tumorigenesis, the temporal relationship between oncogene signaling, in vivo tumor formation, and glycolytic pathway activity is poorly understood. Moreover, how glycolytic pathways are altered as tumors regress remains unknown. Here, we use a switchable model of Myc-driven liver cancer, along with hyperpolarized (13)C-p ...[more]

PMID: 21723511

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