Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

45 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Estrogen receptor regulation of the social transcriptome

ABSTRACT: We investigated the gene regulatory network of the estrogen receptor in the preoptic region of dominant and subordinate male Astatotilapia burtoni. We compared dominant males receiving either an ER antagonist or vehicle control and seperately compared subordinate males receiving either an ER antagonist or control. 8.25% of all genes examined changed expression in DOMs in comparison to only 0.56% in SUBs. Two separate loops (one for dominant males and one for subordinate males) with dye-swap containing 16 arrays each from 2 sources (an ER antagonist treated animal or a vehicle treated animal)

ORGANISM(S): Haplochromis burtoni

SUBMITTER: Lauren O'Connell

PROVIDER: E-GEOD-28508 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Estrogen receptor regulation of the social transcriptome

Project description:We investigated the gene regulatory network of the estrogen receptor in the preoptic region of dominant and subordinate male Astatotilapia burtoni. We compared dominant males receiving either an ER antagonist or vehicle control and seperately compared subordinate males receiving either an ER antagonist or control. 8.25% of all genes examined changed expression in DOMs in comparison to only 0.56% in SUBs.

2011-04-13 | GSE28508 | GEO

Extraction of Total RNA from Single Neurons: Mauthner-cell Cytoplasm vs. Whole Brain

Project description:Astatotilapia burtoni males change their startle behavior in accordance with changes in a socially-mediated phenotypic state. When dominant (DOM), males are brightly colored, isolated from the school and startle readily in response to an auditory pulse. When subordinate (SUB), males are cryptically colored, school and startle less frequently. We hypothesize that the difference in startle responsiveness is governed by serotonergic modulation on the command-like Mauthner cells (M-cells). We used the selective serotonin receptor subtype 2 (5HTR2) antagonist ketanserin to show that serotonin receptors can modulate startle frequency and that the behavioral difference correlates with differences in electrophysiological recordings of M-cells. Specifically, SUB males have a higher serotonergic tone and demonstrate, by behavior and electrophysiological properties, increased sensitivity to 5-HT manipulations compared to DOMs. Immunohistochemistry for serotonin is present around the M-cells. Furthermore, we identified serotonin receptor transcripts in A. burtoni and used single-cell transcriptomics to determine the presence or absence of specific receptor subtypes in the M-cells. Microarray analysis of M-cell samples shows that the neuron’s transcriptome is relatively stable in individuals of different social status. These results are consistent with a role for serotonin in modulating the behavioral response to sensory stimuli in an ecologically-relevant manner through inhibitory interneurons, which establish the membrane resistance of the M-cell before the auditory stimulus. Simple loop with dye-swap containing 6 arrays from 2 sources with 3 biological replicates per source (3 M-cell and 3 whole brain samples).

2010-12-23 | E-GEOD-26277 | biostudies-arrayexpress

Hormonal and stress-response pathways regulate naked mole rat eusociality

Project description:Naked mole rats live in eusocial colonies where subordinates help a single dominant female and a few males to breed. We investigated the genome-wide regulatory mechanisms underlying their reproductive division of labor by examining brain and gonad transcriptomes and DNA-methylomes. Subtle expression differences were observed between brains of dominants and subordinates, but differentially expressed genes clustered consistently in a module with similar function for both sexes. Gonadotropin-releasing hormone (GNRH1) was central in this module and linked with stress-response genes such as neuropeptide Y and corticotrophin-releasing hormone. Breeder-subordinate modifications in DNA methylation were substantial in male brains and associated with the GNRH1 module. The GNRH1-regulated estrogen synthesis pathway was completely blocked in subordinate ovaries and sperm-related genes were significantly down-regulated in subordinate testes. Our results indicate that reproductive suppression is based on hormonal- and stress-related control by the dominant female, but with significant differences in molecular mechanisms between males and females.

2017-03-30 | GSE67542 | GEO

Social status and ejaculate investment in the house mouse c4pr_liv

Project description:Sperm competition theory predicts that males should tailor ejaculates according to their social status. Here we test this in a model vertebrate, the house mouse (Mus musculus domesticus), combining experimental data with a quantitative proteomics analysis of seminal fluid composition. Our analyses reveal that both sperm production and the relative production of proteins found in seminal fluid differ according to social dominance. Notably, whereas dominant males produce and ejaculate more sperm, subordinate males produce greater relative amounts of key proteins used in the formation of copulatory plugs. These findings have important implications for understanding the dynamics and outcome of sperm competition.

2021-09-09 | PXD019727 | Pride

Granulosa cell gene expression profiling of bovine cohort, dominant and subordinate follicles

Project description:Bovine granulosa cells harvested from cohort follicles at 1.3 days after wave emergence, and from dominant and subordinate follicles at 2.6 days after wave emergence. Total RNA extracted from granulosa cells of 4 cohort, 4 dominant and 4 subordinate follicles was pooled for generation of each respective SAGE library. Keywords: other

2005-04-30 | GSE2598 | GEO

Transcription profiling of brain tissue from trout with different dominace status

Project description:Dominance hierarchies of rainbow trout Trout form linear dominance hierarchies where there is a dominant, subdominant and subordinate. The dominant is the most aggressive, followed by the subdominant and the subordinate is the least dominant. Fish were scored on their aggressive performance to calculate position within the hierarchy, and once formed the hierarchies are very stable. Hypothesis: Gene expression is different in the brains of individuals with different dominance status. Six hierarchies containing a dominant, subdominant and subordinate were assessed behaviourally then killed and the brain removed. These were run in a reference based design. Hypothesis: On removal of the dominant the subdominant fish will become dominant and its transcript profile will become similar to the established dominant. The second experiment involved removing the dominant from an established hierarchy and then examining gene expression at 2hr, 48hr and 1 week in the new dominant.

2011-04-05 | E-MAXD-33 | biostudies-arrayexpress

Granulosa cell gene expression profiling of bovine cohort, dominant and subordinate follicles

2005-04-30 | E-GEOD-2598 | biostudies-arrayexpress

Polistes_metricus_dominance_arrays

Project description:This experiment examined ovary gene expression differences between females in primitively eusocial Polistes metricus paper wasps. Specifically, we compared expression patterns between groups of females that differed in their reproductive dominance status. We compared dominant and subordinate foundresses, dominant and subordinate workers, and egg-laying queens. The purpose was to investigate how the social environment and dominance status affect ovary gene expression in individual females.

2014-01-15 | E-MTAB-2191 | biostudies-arrayexpress

Extraction of Total RNA from Single Neurons: Mauthner-cell Cytoplasm vs. Whole Brain

2010-12-23 | GSE26277 | GEO

Differential expression of miRNAs in granulosa cells of bovine preovulatory dominant and subordinate follicles

Project description:The study aimed to uncover differential expression pattern of regulatory microRNAs in bovine granulosa cells derived from preovulatory dominant and subordinate follicles.

2015-03-18 | GSE56002 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data