Estrogen receptor regulation of the social transcriptome
Ontology highlight
ABSTRACT:
ORGANISM(S): Haplochromis burtoni
PROVIDER: GSE28508 | GEO | 2011/04/13
SECONDARY ACCESSION(S): PRJNA138991
REPOSITORIES: GEO
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Project description:We investigated the gene regulatory network of the estrogen receptor in the preoptic region of dominant and subordinate male Astatotilapia burtoni. We compared dominant males receiving either an ER antagonist or vehicle control and seperately compared subordinate males receiving either an ER antagonist or control. 8.25% of all genes examined changed expression in DOMs in comparison to only 0.56% in SUBs. Two separate loops (one for dominant males and one for subordinate males) with dye-swap containing 16 arrays each from 2 sources (an ER antagonist treated animal or a vehicle treated animal)
2011-04-13 | E-GEOD-28508 | biostudies-arrayexpress
Project description:Astatotilapia burtoni males change their startle behavior in accordance with changes in a socially-mediated phenotypic state. When dominant (DOM), males are brightly colored, isolated from the school and startle readily in response to an auditory pulse. When subordinate (SUB), males are cryptically colored, school and startle less frequently. We hypothesize that the difference in startle responsiveness is governed by serotonergic modulation on the command-like Mauthner cells (M-cells). We used the selective serotonin receptor subtype 2 (5HTR2) antagonist ketanserin to show that serotonin receptors can modulate startle frequency and that the behavioral difference correlates with differences in electrophysiological recordings of M-cells. Specifically, SUB males have a higher serotonergic tone and demonstrate, by behavior and electrophysiological properties, increased sensitivity to 5-HT manipulations compared to DOMs. Immunohistochemistry for serotonin is present around the M-cells. Furthermore, we identified serotonin receptor transcripts in A. burtoni and used single-cell transcriptomics to determine the presence or absence of specific receptor subtypes in the M-cells. Microarray analysis of M-cell samples shows that the neuron’s transcriptome is relatively stable in individuals of different social status. These results are consistent with a role for serotonin in modulating the behavioral response to sensory stimuli in an ecologically-relevant manner through inhibitory interneurons, which establish the membrane resistance of the M-cell before the auditory stimulus. Simple loop with dye-swap containing 6 arrays from 2 sources with 3 biological replicates per source (3 M-cell and 3 whole brain samples).
2010-12-23 | E-GEOD-26277 | biostudies-arrayexpress
Project description:the goal of this experiment is to identify the molecular modules that underlie social phenotypes in A. burtoni Keywords: loop design comparison of dominant (T) subordinate (NT) and brooding female (F) phenotypes 6 individuals of each phenotype under normal mixed population conditions
2008-02-27 | GSE10624 | GEO
Project description:Astatotilapia burtoni males change their startle behavior in accordance with changes in a socially-mediated phenotypic state. When dominant (DOM), males are brightly colored, isolated from the school and startle readily in response to an auditory pulse. When subordinate (SUB), males are cryptically colored, school and startle less frequently. We hypothesize that the difference in startle responsiveness is governed by serotonergic modulation on the command-like Mauthner cells (M-cells). We used the selective serotonin receptor subtype 2 (5HTR2) antagonist ketanserin to show that serotonin receptors can modulate startle frequency and that the behavioral difference correlates with differences in electrophysiological recordings of M-cells. Specifically, SUB males have a higher serotonergic tone and demonstrate, by behavior and electrophysiological properties, increased sensitivity to 5-HT manipulations compared to DOMs. Immunohistochemistry for serotonin is present around the M-cells. Furthermore, we identified serotonin receptor transcripts in A. burtoni and used single-cell transcriptomics to determine the presence or absence of specific receptor subtypes in the M-cells. Microarray analysis of M-cell samples shows that the neuron’s transcriptome is relatively stable in individuals of different social status. These results are consistent with a role for serotonin in modulating the behavioral response to sensory stimuli in an ecologically-relevant manner through inhibitory interneurons, which establish the membrane resistance of the M-cell before the auditory stimulus.
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Project description:Naked mole rats live in eusocial colonies where subordinates help a single dominant female and a few males to breed. We investigated the genome-wide regulatory mechanisms underlying their reproductive division of labor by examining brain and gonad transcriptomes and DNA-methylomes. Subtle expression differences were observed between brains of dominants and subordinates, but differentially expressed genes clustered consistently in a module with similar function for both sexes. Gonadotropin-releasing hormone (GNRH1) was central in this module and linked with stress-response genes such as neuropeptide Y and corticotrophin-releasing hormone. Breeder-subordinate modifications in DNA methylation were substantial in male brains and associated with the GNRH1 module. The GNRH1-regulated estrogen synthesis pathway was completely blocked in subordinate ovaries and sperm-related genes were significantly down-regulated in subordinate testes. Our results indicate that reproductive suppression is based on hormonal- and stress-related control by the dominant female, but with significant differences in molecular mechanisms between males and females.
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Project description:Bovine granulosa cells harvested from cohort follicles at 1.3 days after wave emergence, and from dominant and subordinate follicles at 2.6 days after wave emergence. Total RNA extracted from granulosa cells of 4 cohort, 4 dominant and 4 subordinate follicles was pooled for generation of each respective SAGE library. Keywords: other
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Project description:Dominance hierarchies of rainbow trout Trout form linear dominance hierarchies where there is a dominant, subdominant and subordinate. The dominant is the most aggressive, followed by the subdominant and the subordinate is the least dominant. Fish were scored on their aggressive performance to calculate position within the hierarchy, and once formed the hierarchies are very stable. Hypothesis: Gene expression is different in the brains of individuals with different dominance status. Six hierarchies containing a dominant, subdominant and subordinate were assessed behaviourally then killed and the brain removed. These were run in a reference based design. Hypothesis: On removal of the dominant the subdominant fish will become dominant and its transcript profile will become similar to the established dominant. The second experiment involved removing the dominant from an established hierarchy and then examining gene expression at 2hr, 48hr and 1 week in the new dominant.
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Project description:Bovine granulosa cells harvested from cohort follicles at 1.3 days after wave emergence, and from dominant and subordinate follicles at 2.6 days after wave emergence. Total RNA extracted from granulosa cells of 4 cohort, 4 dominant and 4 subordinate follicles was pooled for generation of each respective SAGE library. Keywords: other
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Project description:This experiment examined ovary gene expression differences between females in primitively eusocial Polistes metricus paper wasps. Specifically, we compared expression patterns between groups of females that differed in their reproductive dominance status. We compared dominant and subordinate foundresses, dominant and subordinate workers, and egg-laying queens. The purpose was to investigate how the social environment and dominance status affect ovary gene expression in individual females.
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