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Transcription profiling of human monocyte-derived dendritic cells after treatment with with CpG-oligo D19

ABSTRACT: Human monocyte-derived dendritic cell cultures devoid of contaminating plasmacytoid dendritic cells were treated with the D-type CpG-oligonucleotide D19, with the non-CpG-oligonucleotide control D, or were left untreated. Cells of all treatment groups stemmed from the same donor. 6 h after addition, cells were harvested, and total RNA was prepared for analysis of D19-dependent differential gene expression on Affymetrix HG-U 133A 2.0. Experiment Overall Design: One sample per treatment-group. No replicates.

ORGANISM(S): Homo sapiens

SUBMITTER: Reinhard Wanner 

PROVIDER: E-GEOD-2859 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Human monocyte-derived dendritic cells express TLR9 and react directly to the CpG-A oligonucleotide D19.

Hoene Victoria V   Peiser Matthias M   Wanner Reinhard R  

Journal of leukocyte biology 20060925 6

Oligodeoxynucleotides (ODNs) containing unmethylated CpG exhibit their immunostimulatory activities by binding to TLR. Here, we show that human monocyte-derived dendritic cells (moDC) contain TLR9 protein, surprisingly, in amounts comparable with plasmacytoid DC (pDC). Immature moDC but not mature moDC nor monocytes captured CpG-ODNs. moDC stimulation with the CpG-A ODN D19 up-regulated CD83, CD86, and HLA-DR. Without CD40 ligand costimulation, full maturation was not achieved. D19-stimulated mo  ...[more]

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